Correlation of mRNA and protein levels: Cell type-specific gene expression of cluster designation antigens in the prostate

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Correlation of mRNA and protein levels: Cell type-specific gene expression of cluster designation antigens in the prostate

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dc.contributor.author Pascal, Laura E. en_US
dc.contributor.author True, Lawrence D. en_US
dc.contributor.author Campbell, David S. en_US
dc.contributor.author Deutsch, Eric W. en_US
dc.contributor.author Risk, Michael en_US
dc.contributor.author Coleman, Ilsa M. en_US
dc.contributor.author Eichner, Lillian J. en_US
dc.contributor.author Nelson, Peter S. en_US
dc.contributor.author Liu, Alvin Y. en_US
dc.date.accessioned 2010-05-06T20:07:56Z
dc.date.available 2010-05-06T20:07:56Z
dc.date.issued 2008 en_US
dc.identifier.citation Pascal L, True L, Campbell D, et al. Correlation of mRNA and protein levels: Cell type-specific gene expression of cluster designation antigens in the prostate. BMC Genomics. 2008;9(1):246. en_US
dc.identifier.other 10.1186/1471-2164-9-246 en_US
dc.identifier.uri http://www.biomedcentral.com/1471-2164/9/246 en_US
dc.identifier.uri http://hdl.handle.net/1773/15861
dc.description.abstract Background: Expression levels of mRNA and protein by cell types exhibit a range of correlations for different genes. In this study, we compared levels of mRNA abundance for several cluster designation (CD) genes determined by gene arrays using magnetic sorted and laser-capture microdissected human prostate cells with levels of expression of the respective CD proteins determined by immunohistochemical staining in the major cell types of the prostate - basal epithelial, luminal epithelial, stromal fibromuscular, and endothelial - and for prostate precursor/stem cells and prostate carcinoma cells. Immunohistochemical stains of prostate tissues from more than 50 patients were scored for informative CD antigen expression and compared with cell-type specific transcriptomes. Results: Concordance between gene and protein expression findings based on 'present' vs. 'absent' calls ranged from 46 to 68%. Correlation of expression levels was poor to moderate (Pearson correlations ranged from 0 to 0.63). Divergence between the two data types was most frequently seen for genes whose array signals exceeded background (> 50) but lacked immunoreactivity by immunostaining. This could be due to multiple factors, e.g. low levels of protein expression, technological sensitivities, sample processing, probe set definition or anatomical origin of tissue and actual biological differences between transcript and protein abundance. Conclusion: Agreement between these two very different methodologies has great implications for their respective use in both molecular studies and clinical trials employing molecular biomarkers. en_US
dc.description.sponsorship This work was supported by grant DK63630 and DK069690 from NIDDK. Additional funding came from grants CA85859, CA98699 and CA111244 from NCI, and PM50 GMO76547/Center for Systems Biology. en_US
dc.language.iso en_US en_US
dc.title Correlation of mRNA and protein levels: Cell type-specific gene expression of cluster designation antigens in the prostate en_US
dc.type Article en_US


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