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Detection of MegaTAL-induced HIV pol Mutation By Droplet Digital PCR
Reliable detection of low-level mutations in HIV-1 provirus is still a challenge. Current methods regularly used in laboratories, Surveyor assay and bulk sequencing, only have a limit of detection about 10 ~ 20%. In the Jerome lab, a potential cure for HIV, megaTALs, is under development and a method to evaluate its outcome ...
Development of A Flow Cytometry Assay for Measurement of CD4+ T Cell Proliferation
Measurement of CD4+ T cell function has been widely utilized in numerous studies. In clinical medicine, in vitro tests to measure CD4+ T cell functions such as activation and proliferative capacity have been established for decades. The Ki67 molecule is a nuclear protein present exclusively in proliferating cells. Ki67 protein ...
Comparison of Clinical Features Between Inpatient and Outpatient Cases of Clostridium difficile Infection
The Microbiology Laboratories at Harborview Medical Center and the University of Washington Medical Center evaluated the Biofire FilmArray Gastrointestinal Panel, a multiplex PCR assay to conventional stool culture. The FilmArray can detect both toxin A (tcdA) and toxin B genes (tcdB) in Clostridium difficile. C. difficile ...
Method Development and Validation of Vitamin D2 and Vitamin D3 using Mass Spectrometry
Vitamin D has long been known to maintain bone health by regulating calcium and phosphorous homeostasis. In recent years, scientists have discovered additional physiological roles for vitamin D. The complex interaction between the active vitamin D hormone and its metabolic precursors continues to be a rich area of research. ...
OPTIMIZATION OF FLOW CYTOMETRIC SORTING IN THE HEMATOPATHOLOGY LABORATORY
University of Washington Abstract OPTIMIZATION OF FLOW CYTOMETRIC SORTING IN THE HEMATOPATHOLOGY LABORATORY Anju Thomas Chair of Supervisory Committee: Dr. Jonathan Fromm MD, PhD Department of Laboratory Medicine A subset of neoplasms in Hematopathology show rare neoplastic cells in a background of abundant non-neoplastic ...
MicroRNA Biomarkers Released by Platelet Activation
(2013-11-14)
MicroRNAs are a group of small noncoding RNA molecules that have been implicated in a variety of human diseases. Because of their remarkable stability and abundant quantity in blood, circulating microRNAs are promising as noninvasive biomarkers for diagnosis or prognosis. Platelets are likely to be a substantial contributor ...
Characterizing Aztreonam Resistance in Pseudomonas aeruginosa through Artificial Selection and Whole Genome Sequencing
While much attention has been focused on acquired antibiotic resistance genes, chromosomal mutations may be most important in chronic infections where isolated, persistently infecting lineages experience repeated antibiotic exposure. Here, we used experimental evolution and whole genome sequencing to investigate chromosomally-encoded ...
Establishing Prehospital Transfusion with Airlift Northwest and Harborview Medical Center
Harborview Medical Center (HMC) routinely received patients transferred from outside facilities. These patients would be supported with blood products sent by the transferring facility, and on arrival these products would be discarded due to unvalidated shipping methods. This process put a strain on local blood suppliers and ...
Evaluation of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry for Clinical Microbiology Applications
(2014-04-30)
MALDI-TOF-MS has the ability to streamline identification of microorganisms in the microbiology lab. In this project, we focused on two main aims. First, the implementation of this technology for direct blood culture identification. Within this aim we determined the limit of detection for the protein purification method we ...