Chemical Modification of Cellulose for Biomolecule Capture

ResearchWorks/Manakin Repository

Search ResearchWorks


Advanced Search

Browse

My Account

Statistics

Related Information

Chemical Modification of Cellulose for Biomolecule Capture

Show full item record

Title: Chemical Modification of Cellulose for Biomolecule Capture
Author: Yu, Arthur
Abstract: Paper-based analytical devices are the subject of growing interest for the development of low-cost point-of-care diagnostics, environmental monitoring technologies, and research tools for limited-resource settings. However, there are limited chemistries available for the conjugation of biomolecules to cellulose for use in biomedical applications. Herein divinyl sulfone (DVS) chemistry is demonstrated to covalently immobilize small molecules, proteins and DNA onto the hydroxyl groups of cellulose membranes through nucleophilic addition. Assays on modified cellulose using protein-carbohydrate and protein-glycoprotein interactions as well as oligonucleotide hybridization show that the membrane's bioactivity was specific, dose-dependent, and stable over a long period of time. Use of an inkjet printer to form patterns of biomolecules on DVS-activated cellulose illustrates the adaptability of the DVS functionalization technique to pattern sophisticated designs, with potential applications in cellulose-based lateral flow devices.
Description: Thesis (Master's)--University of Washington, 2012
URI: http://hdl.handle.net/1773/20896
Author requested restriction: Restrict to UW for 6 months -- then make Open Access

Files in this item

Files Size Format View
Yu_washington_0250O_10485.pdf 2.249Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record