Cloning and distribution of galanin-like peptide mRNA in the hypothalamus and pituitary of the macaque

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Cloning and distribution of galanin-like peptide mRNA in the hypothalamus and pituitary of the macaque

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dc.contributor.author Steiner, Robert A. en_US
dc.contributor.author Cunningham, Matthew J. en_US
dc.contributor.author Scarlett, Jarrad M. en_US
dc.date.accessioned 2008-10-17T20:41:18Z
dc.date.available 2008-10-17T20:41:18Z
dc.date.issued 2002-03 en_US
dc.identifier.citation Endocrinology. 2002 Mar;143(3):755-63 en_US
dc.identifier.uri http://hdl.handle.net/1773/4345
dc.description.abstract Galanin-like peptide (GALP) is a newly discovered hypothalamic neuropeptide, which is regulated by leptin and implicated in the regulation of GnRH secretion in the rodent. We searched the human genome database and determined that the human GALP gene comprises six exons, as has been shown for human galanin. We used rapid amplification of cDNA ends to clone a full-length cDNA (802 bp) of the macaque homologue of GALP and found it to be highly conserved between human and macaque at both the nucleotide (93%) and peptide (94%) levels. Mature GALP is predicted to be 60 amino acids in the macaque as in other species, and the region of GALP (9-21) that shows homology to the N-terminal 13 amino acids of galanin is perfectly conserved. We mapped the distribution of GALP mRNA in the hypothalamus and pituitary of the macaque by in situ hybridization and observed that, as in rodent species, the expression of GALP mRNA is confined to the arcuate nucleus, median eminence, and neurohypophysis. Using double-label in situ hybridization, we found that nearly all (98%) GALP mRNA-expressing cells in the arcuate nucleus also express mRNA for the long form of the leptin receptor. These findings suggest that a leptin-GALP signaling pathway exists in a primate species. en_US
dc.language.iso en_US en_US
dc.publisher Endocrine Society en_US
dc.subject mRNA en_US
dc.subject hypothalamus en_US
dc.subject pituitary en_US
dc.subject macaque en_US
dc.subject galanin-like peptide en_US
dc.subject.mesh Molecular Sequence Data en_US
dc.subject.mesh Macaca nemestrina en_US
dc.subject.mesh Pituitary Gland, metabolism en_US
dc.subject.mesh RNA, Messenger, biosynthesis en_US
dc.subject.mesh Nerve Tissue Proteins, biosynthesis, metabolism en_US
dc.subject.mesh Amino Acid Sequence en_US
dc.subject.mesh Galanin-Like Peptide en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Animals en_US
dc.subject.mesh RNA Probes en_US
dc.subject.mesh Humans en_US
dc.subject.mesh Hypothalamus, metabolism en_US
dc.subject.mesh Gene Amplification en_US
dc.subject.mesh Base Sequence en_US
dc.subject.mesh Cloning, Molecular en_US
dc.title Cloning and distribution of galanin-like peptide mRNA in the hypothalamus and pituitary of the macaque en_US
dc.type Article en_US


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