Fanconi anemia complementation group C is required for proliferation of murine primordial germ cells

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Fanconi anemia complementation group C is required for proliferation of murine primordial germ cells

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dc.contributor.author Nadler, Jessica J. en_US
dc.contributor.author Braun, Robert E. en_US
dc.date.accessioned 2008-10-17T20:42:20Z
dc.date.available 2008-10-17T20:42:20Z
dc.date.issued 2000-07 en_US
dc.identifier.citation Genesis. 2000 Jul;27(3):117-23 en_US
dc.identifier.uri http://hdl.handle.net/1773/4413
dc.description.abstract Fanconi anemia is a polygenic trait hypothesized to be a DNA damage repair disease. We show that all three Fanconi anemia loci that have been cloned are expressed in the embryonic gonad during the period of primordial germ cell proliferation. Mice mutant for the Fanconi anemia complementation group C locus (Fancc) have reduced germ cell numbers as early as embryonic day E12.5, suggesting the Fancc protein functions prior to meiosis in both sexes. Depletion in the mutant occurs at a time when all three loci would be expressed in a wild-type gonad, implying a function in the early germline. Determination of the mitotic index of primordial germ cells by BrdU incorporation shows that germ cells in Fancc(-/-) mice proliferate significantly more slowly than littermate controls. This study demonstrates Fancc is required for mitotic proliferation of primordial germ cells. en_US
dc.language.iso en_US en_US
dc.publisher Wiley-Liss en_US
dc.subject mice en_US
dc.subject gene expression en_US
dc.subject primordial germ cells en_US
dc.subject Fanconi anemia en_US
dc.subject.mesh Meiosis en_US
dc.subject.mesh Testis, embryology en_US
dc.subject.mesh Male en_US
dc.subject.mesh Mice, Inbred C57BL en_US
dc.subject.mesh Mitotic Index en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Cell Division en_US
dc.subject.mesh Cell Cycle Proteins en_US
dc.subject.mesh Fanconi Anemia Complementation Group C Protein en_US
dc.subject.mesh Pregnancy en_US
dc.subject.mesh Nuclear Proteins en_US
dc.subject.mesh Ovary, embryology en_US
dc.subject.mesh DNA-Binding Proteins en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Mice, Mutant Strains en_US
dc.subject.mesh Female en_US
dc.subject.mesh Gene Deletion en_US
dc.subject.mesh Fanconi Anemia Complementation Group Proteins en_US
dc.subject.mesh Ovum, cytology, physiology en_US
dc.subject.mesh Embryonic and Fetal Development, genetics en_US
dc.subject.mesh Spermatozoa, cytology, physiology en_US
dc.subject.mesh Fanconi Anemia, genetics en_US
dc.subject.mesh Reverse Transcriptase Polymerase Chain Reaction en_US
dc.subject.mesh Proteins, genetics, metabolism en_US
dc.title Fanconi anemia complementation group C is required for proliferation of murine primordial germ cells en_US
dc.type Article en_US


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