Cloning and characterization of the mouse interleukin enhancer binding factor 3 (Ilf3) homolog in a screen for RNA binding proteins

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Cloning and characterization of the mouse interleukin enhancer binding factor 3 (Ilf3) homolog in a screen for RNA binding proteins

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dc.contributor.author Disteche, Christine en_US
dc.contributor.author Edelhoff, Susanne en_US
dc.contributor.author Braun, Robert E. en_US
dc.contributor.author Buass, F. William en_US
dc.contributor.author Lee, Keesook en_US
dc.date.accessioned 2008-10-17T20:43:04Z
dc.date.available 2008-10-17T20:43:04Z
dc.date.issued 1999-05 en_US
dc.identifier.citation Mamm Genome. 1999 May;10(5):451-6 en_US
dc.identifier.uri http://hdl.handle.net/1773/4456
dc.description.abstract In a screen for RNA-binding proteins expressed during murine spermatogenesis, we have identified a cDNA that encodes a protein of 911 amino acids that contains two copies of the double-stranded RNA-binding motif and has 80% identity with human Interleukin Enhancer Binding Factor 3 (ILF3). Linkage and cytogenetic analyses localized the Ilf3 cDNA to a portion of mouse Chr 9, which shows conserved synteny with a region of human Chr 19 where the human ILF3 gene had been previously localized, supporting that we had cloned the murine homolog of ILF3. Northern analysis indicated the Ilf3 gene is ubiquitously expressed in mouse adult tissues with high levels of expression in the brain, thymus, testis, and ovary. Polyclonal antibodies detected multiple protein species in a subset of the tissues expressing Ilf3 RNA. Immunoreactive species are present at high levels in the thymus, testis, ovary, and the spleen to a lesser extent. The high degree of sequence similarity between the mouse ILF3 protein and other dsRNA binding motif-containing proteins suggests a role in RNA metabolism, while the differential expression indicates the mouse ILF3 protein predominantly functions in tissues containing developing lymphocyte and germ cells. en_US
dc.language.iso en_US en_US
dc.publisher Springer-Verlag en_US
dc.subject cloning en_US
dc.subject mouse en_US
dc.subject RNA en_US
dc.subject ILF3 en_US
dc.subject.mesh Humans en_US
dc.subject.mesh DNA-Binding Proteins, genetics en_US
dc.subject.mesh Molecular Sequence Data en_US
dc.subject.mesh Nuclear Factor 90 Proteins en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Base Sequence en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Nuclear Proteins en_US
dc.subject.mesh RNA-Binding Proteins, genetics en_US
dc.subject.mesh 3' Untranslated Regions en_US
dc.subject.mesh Transcription Factors, genetics en_US
dc.subject.mesh NFATC Transcription Factors en_US
dc.subject.mesh Cloning, Molecular en_US
dc.subject.mesh DNA, Complementary en_US
dc.subject.mesh Chromosome Mapping en_US
dc.subject.mesh Research Support, Non-U.S. Gov't en_US
dc.subject.mesh Amino Acid Sequence en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Sequence Homology, Amino Acid en_US
dc.title Cloning and characterization of the mouse interleukin enhancer binding factor 3 (Ilf3) homolog in a screen for RNA binding proteins en_US
dc.type Article en_US


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