Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse

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Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse

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dc.contributor.author Johnson, Laura L. en_US
dc.contributor.author Clifton, Donald K. en_US
dc.contributor.author Li, Dorothy en_US
dc.contributor.author Steiner, Robert A. en_US
dc.contributor.author Teklemichael, Dawit N. en_US
dc.contributor.author Krasnow, Stephanie M. en_US
dc.contributor.author Jureus, Anders en_US
dc.contributor.author Cunningham, Matthew J. en_US
dc.date.accessioned 2008-10-17T20:44:08Z
dc.date.available 2008-10-17T20:44:08Z
dc.date.issued 2001-12 en_US
dc.identifier.citation Endocrinology. 2001 Dec;142(12):5140-4 en_US
dc.identifier.uri http://hdl.handle.net/1773/4504
dc.description.abstract Galanin-like peptide (GALP) is a newly discovered molecule whose expression in the brain is confined to the arcuate nucleus and median eminence. In the rat, cellular levels of GALP mRNA are reduced by fasting and reversed by peripheral administration of leptin. The purpose of this investigation was 1) to clone and map the distribution of GALP mRNA in the brain of the mouse; 2) to compare the pattern and magnitude of GALP mRNA expression in the leptin-deficient obese (ob/ob) mouse with that of wild-type controls; and 3) to examine the effects of leptin delivered into the brain on the expression of GALP mRNA in the ob/ob mouse. We report the sequence of a mouse GALP cDNA and show that GALP mRNA is expressed in the arcuate nucleus, median eminence, infundibular stalk, and the neurohypophysis of this species. The expression of GALP mRNA in the brain was markedly reduced in the ob/ob mice, compared with wild-type animals. Intracerebroventricular infusion of leptin to ob/ob mice increased both the number of GALP mRNA-expressing neurons and their content of GALP mRNA, compared with vehicle-treated controls. These observations demonstrate that GALP mRNA is induced by leptin through a direct action on the brain. en_US
dc.language.iso en_US en_US
dc.publisher Endocrine Society en_US
dc.subject GALP en_US
dc.subject leptin en_US
dc.subject.mesh Male en_US
dc.subject.mesh Leptin, deficiency en_US
dc.subject.mesh Mice, Inbred C57BL en_US
dc.subject.mesh Obesity, genetics en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Cloning, Molecular en_US
dc.subject.mesh Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject.mesh RNA, Messenger, metabolism en_US
dc.subject.mesh Reference Values en_US
dc.subject.mesh Tissue Distribution en_US
dc.subject.mesh Nerve Tissue Proteins, genetics, metabolism en_US
dc.subject.mesh Galanin-Like Peptide en_US
dc.subject.mesh Hypothalamus, metabolism en_US
dc.subject.mesh DNA, Complementary, genetics en_US
dc.subject.mesh Research Support, Non-U.S. Gov't en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Brain, metabolism en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.title Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse en_US
dc.type Article en_US

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