The attached dataset is a collection of videos taken in an agarose gel model of
the brain in order to examine the differences in diffusive behavior between 100
nm carboxyl-modified polystyrene latex nanoparticles (PS-COOH) and PEGylated
polystyrene latex nanoparticles (PS-PEG).  The data presented are analyzed in
the paper submitted to the Journal of Colloids and Surfaces B: Biointerfaces
"Colloidal stability as a determinant of nanoparticle behavior in the brain" by
Chad Curtis, Dorsa Toghani, Benjamin Wong, and Elizabeth Nance.

 Subject area: Chemical engineering
 More specific subject area: Nanoparticles, diffusion, fluorescent microscopy
 Type of data: TIF files
 How data was acquired: Fluorescent microscopy
 Data format: Compressed
 Data source and location: Seattle, Washington, USA

 Data acquisition
 ----------------

 0.4% agarose gels in  artificial cerebrospinal fluid (ACSF) (CaCl2
 concentrations of 0, 3, 5, and 10 mM, pH 7.2) were prepared by adding 40 mg
 agarose (low EEO, gel point for 1.5% gel, 36±1.5°C, MilliporeSigma) to ACSF.
 The agarose-ACSF mixture was heated to 99°C and mixed well until the agarose
 was completely dissolved, as indicated by no visible particulates in the
 solution. 400 μL agarose-ACSF was added to a well in an 8-well imaging plate
 and PS-COOH or PS-PEG nanoparticles were quickly added and mixed until evenly
 distributed to achieve a concentration of 0.005% solids. Four samples were
 prepared per particle type-condition combination. The prepared gels were
 allowed to set overnight at room temperature. Prior to imaging, the gels were
 allowed to incubate at 37°C and 5% CO2 for 30 min.

 Five videos were collected per well with four wells at 100 Hz and 40x
 magnification for 6.5 s via fluorescent microscopy using a cMOS camera
 (Hamamatsu Photonics, Bridgewater, NJ) mounted on a confocal microscope (Nikon
 Instruments, Melville, NY). Videos are attached as ND2 files containing all
 original metadata.  Videos are 2048 pixels x 2044 pixels (0.16 μm/pixel) and
 651 frames (100.02 frames per second).

 Videos are organized into folders by the experimental conditions in which they
 were collected (0mM, 3mM, 5mM, and 10mM CaCl2).  Files are named according to
 the structure "Channel_Functionality_Temp_pH_Well_Video.nd2" with the following
 parameters:

      * Channel: RED
      * Functionality: PEG, nPEG
      * Temp: 37C
      * pH: 72
      * Well: S1, S1, S3, S4
      * Video: 1, 2, 3, 4, 5

To note, due to errors in data acquisition, a few of the files of the given
naming structure we not successfully acquired.

      * 0mM/RED_nPEG_37C_72pH_S4_2.nd2
      * 5mM/RED_nPEG_37C_72pH_S4_2.nd2
      * 5mM/RED_nPEG_37C_72pH_S4_4.nd2

Original ND2 datafiles can be provided upon request.