Thesis Movie Legend

All movies' speed is 1 minute real time per second movie time except for 4A and 4B, which are at 45 seconds real
time per second movie time.

1A:  Representative DAM1-765 cell at 23C.  Cell is labeled with GFP-TUB1 and SPC110-Cherry.
This cell is typical of DAM1-765 cells in having a uniform sheath of GFP-TUB1 directly between the two poles.
(WT cells form two GFP-TUB1 foci connected by a dimmer stretch of GFP-TUB1).

1B:  Representative DAM1-765 kar3-8 cell at 23C.  Cell is labeled as in movie 1A.
Tubulin does not remain directly between the two poles, but instead frequently collapses outwards in a V shape.


2: A field of asynchronous DAM1-765 kar3-8 cells at 37C.  Cells are labeled as in movie 1A.  Note abundance of
cells whose poles are adjacent to each other with microtubules extending off to one side.  These adjacent poles
rarely separate.


3A:  Asynchronous WT cells labeled with SPC110-Cherry and GFP12LacI12 bound to CEN3::33LacO.  Two metaphase cells 
are visible.  Note clear, stable separation of CEN3 GFP spots.

3B:  Asynchronous cik1-12 DAM1-765 [CEN DAM1] cells labeled with the same labeling.  Note cell with short spindle 
whose CEN3 GFP spots separate temporarily and then collapse back together repeatedly.