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Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule
(2004)
The engineering of green fluorescent protein (GFP) fusion constructs in order to visibly tag a protein of interest has become a commonly used cell biology technique. Although caveats to this
approach are obvious, literature reports in which the chimeric molecule behaves differently than the native molecule are scant. This ...