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dc.contributor.authorViratyosin, Wasnaen_US
dc.contributor.authorCampbell, Lee Annen_US
dc.contributor.authorKuo, Cho-Chouen_US
dc.contributor.authorRockey, Daniel D.en_US
dc.date.accessioned2010-04-21T15:48:20Z
dc.date.available2010-04-21T15:48:20Z
dc.date.issued2002en_US
dc.identifier.citationViratyosin W, Campbell L, Kuo C, Rockey D. Intrastrain and interstrain genetic variation within a paralogous gene family in Chlamydia pneumoniae. BMC Microbiology. 2002;2(1):38.en_US
dc.identifier.other10.1186/1471-2180-2-38en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2180/2/38en_US
dc.identifier.urihttp://hdl.handle.net/1773/15715
dc.description.abstractBackground: Chlamydia pneumoniae causes human respiratory diseases and has recently been associated with atherosclerosis. Analysis of the three recently published C. pneumoniae genomes has led to the identification of a new gene family (the Cpn 1054 family) that consists of 11 predicted genes and gene fragments. Each member encodes a polypeptide with a hydrophobic domain characteristic of proteins localized to the inclusion membrane. Results: Comparative analysis of this gene family within the published genome sequences provided evidence that multiple levels of genetic variation are evident within this single collection of paralogous genes. Frameshift mutations are found that result in both truncated gene products and pseudogenes that vary among isolates. Several genes in this family contain polycytosine (polyC) tracts either upstream or within the terminal 5' end of the predicted coding sequence. The length of the polyC stretch varies between paralogous genes and within single genes in the three genomes. Sequence analysis of genomic DNA from a collection of 12 C. pneumoniae clinical isolates was used to determine the extent of the variation in the Cpn 1054 gene family. Conclusions: These studies demonstrate that sequence variability is present both among strains and within strains at several of the loci. In particular, changes in the length of the polyC tract associated with the different Cpn 1054 gene family members are common within each tested C. pneumoniae isolate. The variability identified within this newly described gene family may modulate either phase or antigenic variation and subsequent physiologic diversity within a C. pneumoniae population.en_US
dc.description.sponsorshipU.S.P.H.S. Awards # AI42869 and AI48769, and the Oregon State University Department of Microbiology N.L. Tartar Award Program.en_US
dc.language.isoen_USen_US
dc.titleIntrastrain and interstrain genetic variation within a paralogous gene family in Chlamydia pneumoniaeen_US
dc.typeArticleen_US


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