Independent centromere formation in a capricious, gene-free domain of chromosome 13q21 in Old World monkeys and pigs

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Independent centromere formation in a capricious, gene-free domain of chromosome 13q21 in Old World monkeys and pigs

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dc.contributor.author Cardone, Maria Francesca en_US
dc.contributor.author Alonso, Alicia en_US
dc.contributor.author Pazienza, Michele en_US
dc.contributor.author Ventura, Mario en_US
dc.contributor.author Montermurro, Gabriella en_US
dc.contributor.author Carbone, Lucia en_US
dc.contributor.author De Jong, Pieter J. en_US
dc.contributor.author Stanyon, Roscoe en_US
dc.contributor.author D'Addabbo, Pietro en_US
dc.contributor.author Archidiacono, Nicoletta en_US
dc.contributor.author She, Xinwei en_US
dc.contributor.author Eichler, Evan E. en_US
dc.contributor.author Warburton, Peter E. en_US
dc.contributor.author Rocchi, Mariano en_US
dc.date.accessioned 2010-04-21T15:50:58Z
dc.date.available 2010-04-21T15:50:58Z
dc.date.issued 2006 en_US
dc.identifier.citation Cardone M, Alonzo A, Pazienza M, et al. Independent centromere formation in a capricious, gene-free domain of chromosome 13q21 in Old World monkeys and pigs. Genome Biology. 2006;7(10):R91. en_US
dc.identifier.other 10.1186/gb-2006-7-10-r91 en_US
dc.identifier.uri http://genomebiology.com/2006/7/10/R91 en_US
dc.identifier.uri http://hdl.handle.net/1773/15735
dc.description.abstract Background: Evolutionary centromere repositioning and human analphoid neocentromeres occurring in clinical cases are, very likely, two stages of the same phenomenon whose properties still remain substantially obscure. Chromosome 13 is the chromosome with the highest number of neocentromeres. We reconstructed the mammalian evolutionary history of this chromosome and characterized two human neocentromeres at 13q21, in search of information that could improve our understanding of the relationship between evolutionarily new centromeres, inactivated centromeres, and clinical neocentromeres. Results: Chromosome 13 evolution was studied, using FISH experiments, across several diverse superordinal phylogenetic clades spanning >100 million years of evolution. The analysis revealed exceptional conservation among primates (hominoids, Old World monkeys, and New World monkeys), Carnivora (cat), Perissodactyla (horse), and Cetartiodactyla (pig). In contrast, the centromeres in both Old World monkeys and pig have apparently repositioned independently to a central location (13q21). We compared these results to the positions of two human 13q21 neocentromeres using chromatin immunoprecipitation and genomic microarrays. Conclusion: We show that a gene-desert region at 13q21 of approximately 3.9 Mb in size possesses an inherent potential to form evolutionarily new centromeres over, at least, approximately 95 million years of mammalian evolution. The striking absence of genes may represent an important property, making the region tolerant to the extensive pericentromeric reshuffling during subsequent evolution. Comparison of the pericentromeric organization of chromosome 13 in four Old World monkey species revealed many differences in sequence organization. The region contains clusters of duplicons showing peculiar features. en_US
dc.description.sponsorship MIUR (Ministero Italiano della Universita' e della Ricerca; Cluster C03, Prog. L.488/92), European Commission (INPRIMAT, QLRI-CT-2002-01325), and NIH R01 GM 061150 and R01 GM058815. en_US
dc.language.iso en_US en_US
dc.title Independent centromere formation in a capricious, gene-free domain of chromosome 13q21 in Old World monkeys and pigs en_US
dc.type Article en_US


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