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dc.contributor.authorTill, Bradley J.en_US
dc.contributor.authorReynolds, Steven H.en_US
dc.contributor.authorWeil, Clifforden_US
dc.contributor.authorSpringer, Nathanen_US
dc.contributor.authorBurtner, Chrisen_US
dc.contributor.authorYoung, Kimen_US
dc.contributor.authorBowers, Elisabethen_US
dc.contributor.authorCodomo, Christine A.en_US
dc.contributor.authorEnns, Linda C.en_US
dc.contributor.authorOdden, Anthony R.en_US
dc.contributor.authorGreene, Elizabeth A.en_US
dc.contributor.authorComail, Lucaen_US
dc.contributor.authorHenikoff, Stevenen_US
dc.date.accessioned2010-04-21T15:51:22Z
dc.date.available2010-04-21T15:51:22Z
dc.date.issued2004en_US
dc.identifier.citationTill B, Reynolds S, Weil C, et al. Discovery of induced point mutations in maize genes by TILLING. BMC Plant Biology. 2004;4(1):12.en_US
dc.identifier.other10.1186/1471-2229-4-12en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2229/4/12en_US
dc.identifier.urihttp://hdl.handle.net/1773/15738
dc.description.abstractBackground: Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern genomics era; however, the most powerful methods are generally restricted to a few model organisms. Previously, we introduced a reverse-genetic strategy with the potential for general applicability to organisms that lack well-developed genetic tools. Our TILLING (Targeting Induced Local Lesions IN Genomes) method uses chemical mutagenesis followed by screening for single-base changes to discover induced mutations that alter protein function. TILLING was shown to be an effective reverse genetic strategy by the establishment of a highthroughput TILLING facility and the delivery of thousands of point mutations in hundreds of Arabidopsis genes to members of the plant biology community. Results: We demonstrate that high-throughput TILLING is applicable to maize, an important crop plant with a large genome but with limited reverse-genetic resources currently available. We screened pools of DNA samples for mutations in 1-kb segments from 11 different genes, obtaining 17 independent induced mutations from a population of 750 pollen-mutagenized maize plants. One of the genes targeted was the DMT102 chromomethylase gene, for which we obtained an allelic series of three missense mutations that are predicted to be strongly deleterious. Conclusions: Our findings indicate that TILLING is a broadly applicable and efficient reversegenetic strategy. We are establishing a public TILLING service for maize modeled on the existing Arabidopsis TILLING Project.en_US
dc.description.sponsorshipThis work was supported by a grant from the Plant Genome Research Project of the National Science Foundation.en_US
dc.language.isoen_USen_US
dc.titleDiscovery of induced point mutations in maize genes by TILLINGen_US
dc.typeArticleen_US


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