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Discovery of induced point mutations in maize genes by TILLING

Show simple item record Till, Bradley J. en_US Reynolds, Steven H. en_US Weil, Clifford en_US Springer, Nathan en_US Burtner, Chris en_US Young, Kim en_US Bowers, Elisabeth en_US Codomo, Christine A. en_US Enns, Linda C. en_US Odden, Anthony R. en_US Greene, Elizabeth A. en_US Comail, Luca en_US Henikoff, Steven en_US 2010-04-21T15:51:22Z 2010-04-21T15:51:22Z 2004 en_US
dc.identifier.citation Till B, Reynolds S, Weil C, et al. Discovery of induced point mutations in maize genes by TILLING. BMC Plant Biology. 2004;4(1):12. en_US
dc.identifier.other 10.1186/1471-2229-4-12 en_US
dc.identifier.uri en_US
dc.description.abstract Background: Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern genomics era; however, the most powerful methods are generally restricted to a few model organisms. Previously, we introduced a reverse-genetic strategy with the potential for general applicability to organisms that lack well-developed genetic tools. Our TILLING (Targeting Induced Local Lesions IN Genomes) method uses chemical mutagenesis followed by screening for single-base changes to discover induced mutations that alter protein function. TILLING was shown to be an effective reverse genetic strategy by the establishment of a highthroughput TILLING facility and the delivery of thousands of point mutations in hundreds of Arabidopsis genes to members of the plant biology community. Results: We demonstrate that high-throughput TILLING is applicable to maize, an important crop plant with a large genome but with limited reverse-genetic resources currently available. We screened pools of DNA samples for mutations in 1-kb segments from 11 different genes, obtaining 17 independent induced mutations from a population of 750 pollen-mutagenized maize plants. One of the genes targeted was the DMT102 chromomethylase gene, for which we obtained an allelic series of three missense mutations that are predicted to be strongly deleterious. Conclusions: Our findings indicate that TILLING is a broadly applicable and efficient reversegenetic strategy. We are establishing a public TILLING service for maize modeled on the existing Arabidopsis TILLING Project. en_US
dc.description.sponsorship This work was supported by a grant from the Plant Genome Research Project of the National Science Foundation. en_US
dc.language.iso en_US en_US
dc.title Discovery of induced point mutations in maize genes by TILLING en_US
dc.type Article en_US

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