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Interaction of an atypical Plasmodium falciparum ETRAMP with human apolipoproteins

Show simple item record Vignali, Marissa en_US McKinlay, Anastasia en_US LaCount, Douglas J. en_US Chettier, Rakesh en_US Bell, Russell en_US Sahasrabudhe, Sudhir en_US Hughes, Robert E. en_US Fields, Stanley en_US 2010-04-21T15:51:47Z 2010-04-21T15:51:47Z 2008 en_US
dc.identifier.citation Vignali M, McKinlay A, LaCount D, et al. Interaction of an atypical Plasmodium falciparum ETRAMP with human apolipoproteins. Malaria Journal. 2008;7(1):211. en_US
dc.identifier.other 10.1186/1475-2875-7-211 en_US
dc.identifier.uri en_US
dc.description.abstract Background: In order to establish a successful infection in the human host, the malaria parasite Plasmodium falciparum must establish interactions with a variety of human proteins on the surface of different cell types, as well as with proteins inside the host cells. To better understand this aspect of malaria pathogenesis, a study was conducted with the goal of identifying interactions between proteins of the parasite and those of its human host. Methods: A modified yeast two-hybrid methodology that preferentially selects protein fragments that can be expressed in yeast was used to conduct high-throughput screens with P. falciparum protein fragments against human liver and cerebellum libraries. The resulting dataset was analyzed to exclude interactions that are not likely to occur in the human host during infection. Results: An initial set of 2,200 interactions was curated to remove proteins that are unlikely to play a role in pathogenesis based on their annotation or localization, and proteins that behave promiscuously in the two-hybrid assay, resulting in a final dataset of 456 interactions. A cluster that implicates binding between P. falciparum PFE1590w/ETRAMP5, a putative parasitophorous vacuole membrane protein, and human apolipoproteins ApoA, ApoB and ApoE was selected for further analysis. Different isoforms of ApoE, which are associated with different outcomes of malaria infection, were shown to display differential interactions with PFE1590w. Conclusion: A dataset of interactions between proteins of P. falciparum and those of its human host was generated. The preferential interaction of the P. falciparum PFE1590w protein with the human ApoE e3 and ApoE e4 isoforms, but not the ApoE e2 isoform, supports the hypothesis that ApoE genotype affects risk of malaria infection. The dataset contains other interactions of potential relevance to disease that may identify possible vaccine candidates and drug targets. en_US
dc.description.sponsorship This work was supported in part by grant P50 GM64655 from the NIH. en_US
dc.language.iso en_US en_US
dc.title Interaction of an atypical Plasmodium falciparum ETRAMP with human apolipoproteins en_US
dc.type Article en_US

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