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Molecular and cellular characterization of ABCG2 in the prostate

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dc.contributor.author Pascal, Laura E. en_US
dc.contributor.author Oudes, Asa J. en_US
dc.contributor.author Petersen, Timothy W. en_US
dc.contributor.author Goo, Young Ah en_US
dc.contributor.author Walashek, Laura S. en_US
dc.contributor.author True, Lawrence D. en_US
dc.contributor.author Liu, Alvin Y. en_US
dc.date.accessioned 2010-04-21T15:52:12Z
dc.date.available 2010-04-21T15:52:12Z
dc.date.issued 2007 en_US
dc.identifier.citation Pascal L, Oudes A, Petersen T, et al. Molecular and cellular characterization of ABCG2 in the prostate. BMC Urology. 2007;7(1):6. en_US
dc.identifier.other 10.1186/1471-2490-7-6 en_US
dc.identifier.uri http://www.biomedcentral.com/1471-2490/7/6 en_US
dc.identifier.uri http://hdl.handle.net/1773/15744
dc.description.abstract Background: Identification and characterization of the prostate stem cell is important for understanding normal prostate development and carcinogenesis. The flow cytometry-based side population (SP) technique has been developed to isolate putative adult stem cells in several human tissue types including the prostate. This phenotype is mainly mediated by the ATP-binding cassette membrane transporter ABCG2. Methods: Immunolocalization of ABCG2 was performed on normal prostate tissue obtained from radical prostatectomies. Normal human prostate SP cells and ABCG2+ cells were isolated and gene expression was determined with DNA array analysis and RT-PCR. Endothelial cells were removed by pre-sorting with CD31. Results: ABCG2 positive cells were localized to the prostate basal epithelium and endothelium. ABCG2+ cells in the basal epithelium constituted less than 1% of the total basal cell population. SP cells constituted 0.5�3% of the total epithelial fraction. The SP transcriptome was essentially the same as ABCG2+ and both populations expressed genes indicative of a stem cell phenotype, however, the cells also expressed many genes in common with endothelial cells. Conclusion: These results provide gene expression profiles for the prostate SP and ABCG2+ cells that will be critical for studying normal development and carcinogenesis, in particular as related to the cancer stem cell concept. en_US
dc.description.sponsorship This work was supported by a grant, DK63630, from the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health. en_US
dc.language.iso en_US en_US
dc.title Molecular and cellular characterization of ABCG2 in the prostate en_US
dc.type Article en_US


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