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mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils

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dc.contributor.author Staudinger, Benjamin J. en_US
dc.contributor.author Oberdoerster, Michele A. en_US
dc.contributor.author Lewis, Patrick J. en_US
dc.contributor.author Rosen, Henry en_US
dc.date.accessioned 2010-04-21T15:56:46Z
dc.date.available 2010-04-21T15:56:46Z
dc.date.issued 2002-10-15 en_US
dc.identifier.citation Staudinger BJ, Oberdoerster MA, Lewis PJ, Rosen H. mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils . J Clin Invest. 2002;110(8):1151-1163. en_US
dc.identifier.other 10.1172/JCI15268 en_US
dc.identifier.uri http://www.jci.org/articles/view/15268 en_US
dc.identifier.uri http://hdl.handle.net/1773/15780
dc.description.abstract To gain a better understanding of bacterial responses to complex and hostile environments generated within the neutrophil phagosome, we estimated mRNA abundance, using genomic arrays, in Escherichia coli cells ingested by normal and phagocyte oxidase-deficient human neutrophils. Genes regulated by the oxidant sensing transcription factor OxyR were among those strongly induced upon phagocytosis by normal, but not oxidase-deficient, neutrophils. Several genes related to nitrogen metabolism, especially those regulated by the NtrC and NAC proteins and transcribed via the ς54 alternative sigma factor, were suppressed by both normal and oxidase-deficient neutrophils. A ΔoxyRS mutant strain of E. coli was significantly more susceptible than the parent strain to neutrophil-mediated killing, which suggests that OxyR-regulated gene products contribute a measure of resistance to neutrophil antimicrobial systems. The hypersusceptibility of the ΔoxyRS mutant was attenuated when oxidase-deficient neutrophils were employed, suggesting that much of the protection afforded by the OxyR regulon is against oxidative antimicrobial factors. Expression profiling of phagocytosed bacteria appears to provide useful information about conditions in the phagocytic vacuole and about bacterial defenses mounted in response to this hostile environment. en_US
dc.description.sponsorship Grant AI25606 from the National Institute of Allergy and Infectious Diseases en_US
dc.language.iso en_US en_US
dc.title mRNA expression profiles for Escherichia coli ingested by normal and phagocyte oxidase-deficient human neutrophils en_US


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