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dc.contributor.authorMorrow, Rhoda Ashleyen_US
dc.contributor.authorFriedrich, Daviden_US
dc.contributor.authorMeier, Amaliaen_US
dc.contributor.authorCorey, Lawrenceen_US
dc.date.accessioned2010-04-21T15:59:25Z
dc.date.available2010-04-21T15:59:25Z
dc.date.issued2005en_US
dc.identifier.citationMorrow R, Friedrich D, Meier A, Corey L. Use of "biokit HSV-2 Rapid Assay" to improve the positive predictive value of Focus HerpeSelect HSV-2 ELISA. BMC Infectious Diseases. 2005;5(1):84.en_US
dc.identifier.other10.1186/1471-2334-5-84en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2334/5/84en_US
dc.identifier.urihttp://hdl.handle.net/1773/15801
dc.description.abstractBackground: Commercially available assays to detect antibodies to the herpes simplex virus type 2 (HSV-2)-specific glycoprotein gG-2 have markedly improved serologic diagnosis of HSV-2 infection. However, even tests with high specificity can have low positive predictive values in low prevalence populations. HSV-2 is a chronic, life-long viral infection that requires both medical attention and potential alterations in health care strategy. As such, the concern for false positive diagnoses is high confirmatory testing is routine for other viral serologies such as HIV and hepatitis C. We evaluated such a strategy for HSV-2 serology by using an easily performed commercial test, biokitHSV-2 rapid test ("Biokit"; Biokit USA, Lexington MA) as a confirmatory test for the widely used gG-2 specific serology ("Focus;" HerpeSelect HSV-2 ELISA; Focus Diagnostics, Cypress CA). Methods: We tested 782 sera by Focus HSV-2 ELISA, Biokit, and the current gold standard test, Western blot (WB). Results: The positive predictive value of the Focus HSV-2 ELISA increased from 80.5% to 95.6% when Biokit testing was performed on sera that were initially positive by Focus HSV-2 ELISA. Confirmatory testing increased the specificity markedly among sera with Focus EIA values between 1.1 and 3.5: only 35% of low positive (index values 1.1-3.5) Focus HSV-2 ELISA results confirmed as positive by Biokit and WB compared with 92% of those with index values >3.5. Mathematical modeling of the data resulted in expected positive predictive values over 98% for populations with antibody prevalences typical of clinical practices in the US and Europe. Conclusion: Confirmatory Biokit testing of positive Focus HSV-2 ELISA results is fast, easy, and effective in reducing falsely positive HSV-2 antibody results. Patients, clinicians, and laboratories could benefit from the enhanced specificity of this simple HSV-2 serologic test combination.en_US
dc.description.sponsorshipThis study was supported by NIH grant AI 30731 and The Bill and Melinda Gates Foundation's Partners in Prevention Project Grant #26469.en_US
dc.language.isoen_USen_US
dc.titleUse of "biokit HSV-2 Rapid Assay" to improve the positive predictive value of Focus HerpeSelect HSV-2 ELISAen_US
dc.typeArticleen_US


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