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dc.contributor.authorSavard, Christopher E.en_US
dc.contributor.authorBlinman, Thane A.en_US
dc.contributor.authorChoi, Ho-Soonen_US
dc.contributor.authorLee, Sung-Kooen_US
dc.contributor.authorPandol, Stephen J.en_US
dc.contributor.authorLee, Sum P.en_US
dc.date.accessioned2010-05-06T20:05:22Z
dc.date.available2010-05-06T20:05:22Z
dc.date.issued2002-10-11en_US
dc.identifier.citationSavard C, Blinman T, Choi H, et al. Expression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-alpha by gallbladder epithelial cells: Response to bacterial lipopolysaccharides. BMC Gastroenterology. 2002;2(1):23.en_US
dc.identifier.other10.1186/1471-230X-2-23en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-230X/2/23en_US
dc.identifier.urihttp://hdl.handle.net/1773/15838
dc.description.abstractBackground: In addition to immune cells, many other cell types are known to produce cytokines. Cultured normal mouse gallbladder epithelial cells, used as a model system for gallbladder epithelium, were examined for their ability to express the mRNA of various cytokines and chemokines in response to bacterial lipopolysaccharide. The synthesis and secretion of the tumor necrosis factor-α (TNF-α) protein by these cells was also measured. Results: Untreated mouse gallbladder cells expressed mRNA for TNF-α, RANTES, and macrophage inflammatory protein-2 (MIP-2). Upon treatment with lipopolysaccharide, these cells now produced mRNA for Interleukin-1β (IL-1β), IL-6, monocyte chemoattractant protein-1 (MCP-1), and showed increased expression of TNF-α and MIP-2 mRNA. Untreated mouse gallbladder cells did not synthesize TNF-α protein; however, they did synthesize and secrete TNF-α upon treatment with lipopolysaccharide. Methods: Cells were treated with lipopolysaccharides from 3 strains of bacteria. Qualitative and semi-quantitative RT-PCR, using cytokine or chemokine-specific primers, was used to measure mRNA levels of TNF-α, IL-1β, IL-6, IL-10, KC, RANTES, MCP-1, and MIP-2. TNF-α protein was measured by immunoassays. Conclusion: This research demonstrates that gallbladder epithelial cells in response to lipopolysaccharide exposure can alter their cytokine and chemokine RNA expression pattern and can synthesize and secrete TNF-α protein. This suggests a mechanism whereby gallbladder epithelial cells in vivo may mediate gallbladder secretory function, inflammation and diseases in an autocrine/ paracrine fashion by producing and secreting cytokines and/or chemokines during sepsis.en_US
dc.description.sponsorshipThis work was supported by a grant from the NIH (DK50246) and in part by the Medical Research Service of the Department of Veterans Affairs.en_US
dc.language.isoen_USen_US
dc.titleExpression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-α by gallbladder epithelial cells: Response to bacterial lipopolysaccharidesen_US


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