Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production

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Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production

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dc.contributor.author Soong, Grace en_US
dc.contributor.author Muir, Amanda en_US
dc.contributor.author Gomez, Marisa I. en_US
dc.contributor.author Waks, Jonathan en_US
dc.contributor.author Reddy, Bharat en_US
dc.contributor.author Planet, Paul en_US
dc.contributor.author Singh, Pradeep en_US
dc.contributor.author Kanetko, Yukihiro en_US
dc.contributor.author Wolfgang, Matthew C. en_US
dc.contributor.author Hsiao, Yu-Shan en_US
dc.contributor.author Tong, Liang en_US
dc.contributor.author Prince, Alice en_US
dc.date.accessioned 2010-05-06T20:06:24Z
dc.date.available 2010-05-06T20:06:24Z
dc.date.issued 2006-08-01 en_US
dc.identifier.citation Soong G, Muir A, Gomez MI, et al. Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production. J Clin Invest. 2006;116(8):2297-2305. en_US
dc.identifier.other 10.1172/JCI27920 en_US
dc.identifier.uri http://www.jci.org/articles/view/27920 en_US
dc.identifier.uri http://hdl.handle.net/1773/15847
dc.description.abstract Many respiratory pathogens, including Hemophilus influenzae, Streptococcus pneumoniae, and Pseudomonas aeruginosa, express neuraminidases that can cleave α2,3-linked sialic acids from glycoconjugates. As mucosal surfaces are heavily sialylated, neuraminidases have been thought to modify epithelial cells by exposing potential bacterial receptors. However, in contrast to neuraminidase produced by the influenza virus, a role for bacterial neuraminidase in pathogenesis has not yet been clearly established. We constructed a mutant of P. aeruginosa PAO1 by deleting the PA2794 neuraminidase locus (Δ2794) and tested its virulence and immunostimulatory capabilities in a mouse model of infection. Although fully virulent when introduced i.p., the Δ2794 mutant was unable to establish respiratory infection by i.n. inoculation. The inability to colonize the respiratory tract correlated with diminished production of biofilm, as assessed by scanning electron microscopy and in vitro assays. The importance of neuraminidase in biofilm production was further demonstrated by showing that viral neuraminidase inhibitors in clinical use blocked P. aeruginosa biofilm production in vitro as well. The P. aeruginosa neuraminidase has a key role in the initial stages of pulmonary infection by targeting bacterial glycoconjugates and contributing to the formation of biofilm. Inhibiting bacterial neuraminidases could provide a novel mechanism to prevent bacterial pneumonia. en_US
dc.description.sponsorship NIH grant R01 DK39693. en_US
dc.language.iso en_US en_US
dc.title Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production en_US


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