Cytotoxic T lymphocytes form an antigen-independent ring junction

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Cytotoxic T lymphocytes form an antigen-independent ring junction

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dc.contributor.author Somersalo, Kristina en_US
dc.contributor.author Anikeeva, Nadja en_US
dc.contributor.author Sims, Tasha N. en_US
dc.contributor.author Thomas, V. Kaye en_US
dc.contributor.author Strong, Roland K. en_US
dc.contributor.author Spies, Thomas en_US
dc.contributor.author Lebedeva,n Tatiana en_US
dc.contributor.author Sykulev, Yuri en_US
dc.contributor.author Dustin, Michael L. en_US
dc.date.accessioned 2010-05-06T20:07:04Z
dc.date.available 2010-05-06T20:07:04Z
dc.date.issued 2004-01-01 en_US
dc.identifier.citation Somersalo K, Anikeeva N, Sims TN, et al. Cytotoxic T lymphocytes form an antigen-independent ring junction. J Clin Invest. 2004;113(1):49-57. en_US
dc.identifier.other 10.1172/JCI19337 en_US
dc.identifier.uri http://www.jci.org/articles/view/19337 en_US
dc.identifier.uri http://hdl.handle.net/1773/15853
dc.description.abstract Immunological synapses are organized cell-cell junctions between T lymphocytes and APCs composed of an adhesion ring, the peripheral supramolecular activation cluster (pSMAC), and a central T cell receptor cluster, the central supramolecular activation cluster (cSMAC). In CD8+ cytotoxic T lymphocytes, the immunological synapse is thought to facilitate specific killing by confining cytotoxic agents to the synaptic cleft. We have investigated the interaction of human CTLs and helper T cells with supported planar bilayers containing ICAM-1. This artificial substrate provides identical ligands to CD4+ and CD8+ T cells, allowing a quantitative comparison. We found that cytotoxic T lymphocytes form a ring junction similar to a pSMAC in response to high surface densities of ICAM-1 in the planar bilayer. MICA, a ligand for NKG2D, facilitated the ring junction formation at lower surface densities of ICAM-1. ICAM-1 and MICA are upregulated in tissues by inflammation- and stress-associated signaling, respectively. Activated CD8+ T cells formed fivefold more ring junctions than did activated CD4+ T cells. The ring junction contained lymphocyte function associated antigen-1 and talin, but did not trigger polarization and granule translocation to the interface. This result has specific implications for the mechanism of effective CTL hunting for antigen in tissues. Abnormalities in this process may alter CTL reactivity. en_US
dc.language.iso en_US en_US
dc.title Cytotoxic T lymphocytes form an antigen-independent ring junction en_US


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