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Transcriptomes of human prostate cells

Show simple item record Oudes, Asa J. en_US Campbell, Dave S. en_US Sorensen, Carrie M. en_US Walashek, Laura S. en_US True, Lawrence D. en_US Liu, Alvin Y. en_US 2010-05-06T20:07:49Z 2010-05-06T20:07:49Z 2006 en_US
dc.identifier.citation Oudes A, Campbell D, Sorensen C, et al. Transcriptomes of human prostate cells. BMC Genomics. 2006;7(1):92. en_US
dc.identifier.other 10.1186/1471-2164-7-92 en_US
dc.identifier.uri en_US
dc.description.abstract Background: The gene expression profiles of most human tissues have been studied by determining the transcriptome of whole tissue homogenates. Due to the solid composition of tissues it is difficult to study the transcriptomes of individual cell types that compose a tissue. To overcome the problem of heterogeneity we have developed a method to isolate individual cell types from whole tissue that are a source of RNA suitable for transcriptome profiling. Results: Using monoclonal antibodies specific for basal (integrin [Beta]4), luminal secretory (dipeptidyl peptidase IV), stromal fibromuscular (integrin [alpha] 1), and endothelial (PECAM-1) cells, respectively, we separated the cell types of the prostate with magnetic cell sorting (MACS). Gene expression of MACS-sorted cell populations was assessed with Affymetrix GeneChips. Analysis of the data provided insight into gene expression patterns at the level of individual cell populations in the prostate. Conclusion: In this study, we have determined the transcriptome profile of a solid tissue at the level of individual cell types. Our data will be useful for studying prostate development and cancer progression in the context of single cell populations within the organ. en_US
dc.description.sponsorship NIH grants DK63630, CA85859, and CA98699. en_US
dc.language.iso en_US en_US
dc.title Transcriptomes of human prostate cells en_US
dc.type Article en_US

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