Browsing Laboratory medicine by Issue Date
Now showing items 1-20 of 32
-
Evaluation of a CD4-aptamer for Flow Cytometry-based Enumeration of T-cell Subsets
(2013-07-25)Introduction: Aptamers are engineered oligonucleotides that can be selected to bind with high specificity and affinity. As aptamers are cost-effective (estimated to be ~1,000-fold cheaper than antibodies) and also ... -
MicroRNA Biomarkers Released by Platelet Activation
(2013-11-14)MicroRNAs are a group of small noncoding RNA molecules that have been implicated in a variety of human diseases. Because of their remarkable stability and abundant quantity in blood, circulating microRNAs are promising as ... -
Measurement of Cystatin C in Dried Blood Spot Specimens
(2014-02-24)Dried blood spot (DBS) technologies can be used in a variety of clinical and research settings. Among other advantages, the use of DBS specimens facilitate the incorporation of biomarkers into demographic research, ... -
Evaluation of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry for Clinical Microbiology Applications
(2014-04-30)MALDI-TOF-MS has the ability to streamline identification of microorganisms in the microbiology lab. In this project, we focused on two main aims. First, the implementation of this technology for direct blood culture ... -
Detection of MegaTAL-induced HIV pol Mutation By Droplet Digital PCR
Reliable detection of low-level mutations in HIV-1 provirus is still a challenge. Current methods regularly used in laboratories, Surveyor assay and bulk sequencing, only have a limit of detection about 10 ~ 20%. In the ... -
Development of A Flow Cytometry Assay for Measurement of CD4+ T Cell Proliferation
Measurement of CD4+ T cell function has been widely utilized in numerous studies. In clinical medicine, in vitro tests to measure CD4+ T cell functions such as activation and proliferative capacity have been established ... -
Comparison of Clinical Features Between Inpatient and Outpatient Cases of Clostridium difficile Infection
The Microbiology Laboratories at Harborview Medical Center and the University of Washington Medical Center evaluated the Biofire FilmArray Gastrointestinal Panel, a multiplex PCR assay to conventional stool culture. The ... -
Method Development and Validation of Vitamin D2 and Vitamin D3 using Mass Spectrometry
Vitamin D has long been known to maintain bone health by regulating calcium and phosphorous homeostasis. In recent years, scientists have discovered additional physiological roles for vitamin D. The complex interaction ... -
OPTIMIZATION OF FLOW CYTOMETRIC SORTING IN THE HEMATOPATHOLOGY LABORATORY
University of Washington Abstract OPTIMIZATION OF FLOW CYTOMETRIC SORTING IN THE HEMATOPATHOLOGY LABORATORY Anju Thomas Chair of Supervisory Committee: Dr. Jonathan Fromm MD, PhD Department of Laboratory Medicine A subset ... -
Characterizing Aztreonam Resistance in Pseudomonas aeruginosa through Artificial Selection and Whole Genome Sequencing
While much attention has been focused on acquired antibiotic resistance genes, chromosomal mutations may be most important in chronic infections where isolated, persistently infecting lineages experience repeated antibiotic ... -
Establishing Prehospital Transfusion with Airlift Northwest and Harborview Medical Center
Harborview Medical Center (HMC) routinely received patients transferred from outside facilities. These patients would be supported with blood products sent by the transferring facility, and on arrival these products would ... -
A Comparison of Disc Diffusion and Microbroth Dilution Methods for the Detection of Antibiotic Resistant Subpopulations in Gram Negative Bacilli
Two of the different types of antibiotic susceptibility tests available are the solid media based Kirby Bauer method and the liquid media based microbroth dilution method. The microbroth dilution method has become more ... -
Validation of an ELISA Method for Epstein-Barr Virus Antibodies in Dried Blood Spot Specimens and Correlation of EBV Serology with DNA Detection and Cytomegalovirus Serology
Dried blood spot (DBS) technology offers several advantages over conventional liquid sample types in terms of cost, storage and stability especially in large population-based or field research. The Epstein-Barr virus (EBV) ... -
Regulation of Phenotypic Heterogeneity in Salmonella: Bifunctional Regulators, RcsB and YifA, Shape Flagellar Bistability
Heterogeneous expression of flagellar and invasion genes is crucial for Salmonella virulence. Such a division of labor enables an invasive subpopulation to trigger inflammation in the infected host and alter the gut ... -
Antibody Characterization for use in Clinical Mass Spectrometry
Many biomarkers in human serum are in low abundance. Immunoaffinity enrichment via anti-peptide antibody and analysis via liquid chromatography tandem mass spectrometry (LC-MS/MS) is an attractive workflow that depends on ... -
A Tale of Two Validations: Molecular Diagnostic Assays for the Detection of Clinically Significant Alterations
Laboratory developed tests (LDTs) have become an integral part of laboratory medicine with applications to a variety of complex genetic testing and are at the frontlines of clinical medicine. Proper validation and ... -
Inherited chromosomally integrated HHV-6 demonstrates tissue-specific RNA expression in vivo
Human herpesvirus-6A and 6B (HHV-6A, HHV-6B) are highly prevalent viruses unique in that they, besides Epstein-Barr virus, are the only human herpesviruses capable of chromosomal integration. When a chromosomal integration ... -
Contributions and Evolutionary Potential of ampC Mutations to confer Aztreonam Resistance in Pseudomonas aeruginosa
Aztreonam is a synthetic monobactam antibiotic that is increasingly used for chronic suppressive therapy in Cystic Fibrosis (CF) patients with Pseudomonas aeruginosa airway infection. P. aeruginosa can accumulate mutations ... -
Design and Development of Hemogram, a Web Application for a Remote Examination of Peripheral Blood Smears from Resource-Limited Clinics in Nepal
Examination of peripheral blood smears is a simple medical laboratory test that can provide signicant diagnostic information. Nevertheless, this test is manual, time-consuming, and demands highly experienced medical ...