Coarsening dynamics of domains in lipid membranes

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Coarsening dynamics of domains in lipid membranes

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dc.contributor.advisor Keller, Sarah L. en_US
dc.contributor.author Stanich, Cynthia Ann en_US
dc.date.accessioned 2012-09-13T17:25:51Z
dc.date.available 2012-09-13T17:25:51Z
dc.date.issued 2012-09-13
dc.date.submitted 2012 en_US
dc.identifier.other Stanich_washington_0250E_10316.pdf en_US
dc.identifier.uri http://hdl.handle.net/1773/20612
dc.description Thesis (Ph.D.)--University of Washington, 2012 en_US
dc.description.abstract We investigate isothermal diffusion and growth of micron-scale liquid domains within membranes of free-floating giant unilamellar vesicles (GUVs) with diameters between 80µm and 250µm. Domains appear after a rapid temperature quench, when the membrane is cooled through a miscibility phase transition such that coexisting liquid phases form. In membranes quenched far from a miscibility critical point, circular domains nucleate and then progress within seconds to "late stage" coarsening in which domains grow via two mechanisms: (1) collision and coalescence of liquid domains, and (2) Ostwald ripening. Both mechanisms are expected to yield the same growth exponent, &alpha = 1/3, where domain radius grows as time<super>&alpha</super>. We measure &alpha = 0.28 ± 0.05, in excellent agreement. In membranes close to a miscibility critical point, the two liquid phases in the membrane are bi-continuous. A quench near the critical composition results in rapid changes in morphology of elongated domains. In this case, we measure &alpha = 0.50 ± 0.16, consistent with theory and simulation. en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.subject 2-D phase separation; biophysics; diffusion coefficient; growth exponent; lipid membranes en_US
dc.subject.other Biophysics en_US
dc.subject.other Chemistry en_US
dc.subject.other Chemistry en_US
dc.title Coarsening dynamics of domains in lipid membranes en_US
dc.type Thesis en_US
dc.embargo.terms No embargo en_US


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