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Chemical Modification of Cellulose for Biomolecule Capture

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dc.contributor.advisor Ratner, Daniel M. en_US Yu, Arthur en_US 2012-09-13T17:40:11Z 2013-03-13T11:04:56Z 2012-09-13 2012 en_US
dc.identifier.other Yu_washington_0250O_10485.pdf en_US
dc.description Thesis (Master's)--University of Washington, 2012 en_US
dc.description.abstract Paper-based analytical devices are the subject of growing interest for the development of low-cost point-of-care diagnostics, environmental monitoring technologies, and research tools for limited-resource settings. However, there are limited chemistries available for the conjugation of biomolecules to cellulose for use in biomedical applications. Herein divinyl sulfone (DVS) chemistry is demonstrated to covalently immobilize small molecules, proteins and DNA onto the hydroxyl groups of cellulose membranes through nucleophilic addition. Assays on modified cellulose using protein-carbohydrate and protein-glycoprotein interactions as well as oligonucleotide hybridization show that the membrane's bioactivity was specific, dose-dependent, and stable over a long period of time. Use of an inkjet printer to form patterns of biomolecules on DVS-activated cellulose illustrates the adaptability of the DVS functionalization technique to pattern sophisticated designs, with potential applications in cellulose-based lateral flow devices. en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.rights Copyright is held by the individual authors. en_US
dc.subject biomolecule; cellulose; covalent; diagnostic; paper; sensor en_US
dc.subject.other Biomedical engineering en_US
dc.subject.other Materials Science en_US
dc.subject.other Bioengineering en_US
dc.title Chemical Modification of Cellulose for Biomolecule Capture en_US
dc.type Thesis en_US
dc.embargo.terms Restrict to UW for 6 months -- then make Open Access en_US

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