Show simple item record

dc.contributor.advisorRatner, Daniel M.en_US
dc.contributor.authorYu, Arthuren_US
dc.date.accessioned2012-09-13T17:40:11Z
dc.date.available2013-03-13T11:04:56Z
dc.date.issued2012-09-13
dc.date.submitted2012en_US
dc.identifier.otherYu_washington_0250O_10485.pdfen_US
dc.identifier.urihttp://hdl.handle.net/1773/20896
dc.descriptionThesis (Master's)--University of Washington, 2012en_US
dc.description.abstractPaper-based analytical devices are the subject of growing interest for the development of low-cost point-of-care diagnostics, environmental monitoring technologies, and research tools for limited-resource settings. However, there are limited chemistries available for the conjugation of biomolecules to cellulose for use in biomedical applications. Herein divinyl sulfone (DVS) chemistry is demonstrated to covalently immobilize small molecules, proteins and DNA onto the hydroxyl groups of cellulose membranes through nucleophilic addition. Assays on modified cellulose using protein-carbohydrate and protein-glycoprotein interactions as well as oligonucleotide hybridization show that the membrane's bioactivity was specific, dose-dependent, and stable over a long period of time. Use of an inkjet printer to form patterns of biomolecules on DVS-activated cellulose illustrates the adaptability of the DVS functionalization technique to pattern sophisticated designs, with potential applications in cellulose-based lateral flow devices.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.rightsCopyright is held by the individual authors.en_US
dc.subjectbiomolecule; cellulose; covalent; diagnostic; paper; sensoren_US
dc.subject.otherBiomedical engineeringen_US
dc.subject.otherMaterials Scienceen_US
dc.subject.otherBioengineeringen_US
dc.titleChemical Modification of Cellulose for Biomolecule Captureen_US
dc.typeThesisen_US
dc.embargo.termsRestrict to UW for 6 months -- then make Open Accessen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record