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dc.contributor.advisorSchief, Williamen_US
dc.contributor.authorMenis, Sergeyen_US
dc.date.accessioned2014-02-24T18:27:14Z
dc.date.issued2014-02-24
dc.date.submitted2013en_US
dc.identifier.otherMenis_washington_0250E_12496.pdfen_US
dc.identifier.urihttp://hdl.handle.net/1773/25105
dc.descriptionThesis (Ph.D.)--University of Washington, 2013en_US
dc.description.abstractHuman Immunodeficiency Virus (HIV) and Hepatitis C Virus (HCV) evolved a number of defense strategies to evade protective mechanisms of the immune system. Classical vaccine approaches have failed to elicit a protective response for these targets. Epitope Scaffolding is a theoretically attractive immunogen design strategy, which isolates known protective epitopes from their environments while stabilizing relevant conformations, as defined by a neutralizing antibody. In this work, we extend previous epitope scaffolding methods to scaffold a known protective HCV epitope and introduce preliminary immunization results. Additionally, in light of several mixed, protein and glycan, epitopes described for HIV, we apply the scaffolding strategy to an anti-HIV broadly neutralizing antibody - PG9. Next, using a derivative of an HIV envelope glycoprotein, we describe the development of a novel multimerization platform and illustrate potential applications. Finally, a computational protocol was developed to identify antibody accessible epitopes on flexible, glycosylated proteins.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.rightsCopyright is held by the individual authors.en_US
dc.subject.otherBiochemistryen_US
dc.subject.otherbiological chemistryen_US
dc.titleExploring Epitope-Focused Vaccine Development: Design of Epitope Scaffolds and Nanoparticle Presentation Platforms, and Computational Prediction of Conformational Epitopesen_US
dc.typeThesisen_US
dc.embargo.termsDelay release for 2 years -- then make Open Accessen_US
dc.embargo.lift2016-02-14T18:27:14Z


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