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dc.contributor.advisorBaker, Daviden_US
dc.contributor.authorWang, Yu-Rueien_US
dc.date.accessioned2015-09-29T17:57:57Z
dc.date.submitted2015en_US
dc.identifier.otherWang_washington_0250E_15153.pdfen_US
dc.identifier.urihttp://hdl.handle.net/1773/33597
dc.descriptionThesis (Ph.D.)--University of Washington, 2015en_US
dc.description.abstractSingle-particle cryo-electron microscopy (cryo-EM) has emerged as a powerful tool in structure determination of macromolecular complexes that are not suitable for crystallographic studies. Recent advances in direct electron detectors and image-processing techniques have allowed cryo-EM to reach near-atomic resolution (3–5 Å) from small particles with low or even no symmetry. However, tools to determine structures from cryo-EM maps have lagged behind. In this dissertation we describe approaches that apply Rosetta high-resolution structure prediction methods to model-building from cryo-EM maps. Using knowledge-based information gathered from proteins of known atomic structure, we describe the development of novel tools for de novo model-building from near-atomic resolution cryo-EM maps, and for architecture determination of multi-component macromolecular assemblies from medium- to low-resolution (5–15 Å) cryo- EM maps. We demonstrate that these new computational tools have shown usefulness in determining the structures from newly reconstructed cryo-EM maps, which would otherwise be difficult or impossible for human to do. These methods should enable rapid and reliable structure determination from cryo-EM maps.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.rightsCopyright is held by the individual authors.en_US
dc.subjectCryo-EM; electron density map; Model building; Rosetta; Structure determination; Structure predictionen_US
dc.subject.otherBiochemistryen_US
dc.subject.otherBioinformaticsen_US
dc.subject.otherbiological chemistryen_US
dc.titleProtein Structure Determination from Cryo-electron Microscopy Density Mapsen_US
dc.typeThesisen_US
dc.embargo.termsRestrict to UW for 1 year -- then make Open Accessen_US
dc.embargo.lift2016-09-28T17:57:57Z


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