Cell-specific transcriptional analysis in complex tissues
McKinney, Bonnie Lewis
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Cell-type specific transcriptional analysis can yield important information about the role of a certain cell type in the context of its tissue microenvironment. Previous methods for isolating cell type-specific transcriptional data have included enzymatic or mechanical tissue dispersion followed by magnetic cell sorting (MACS) or fluorescence-activated cell sorting (FACS) and laser capture microdissection. However, these methods have important limitations. Transcriptome analysis by translating ribosome affinity purification (TRAP) allows the researcher to isolate translating mRNA from a genetically defined cell type. Discussed in this review are current methods for translating ribosome affinity purification, caveats of this method, and considerations for successfully completing these studies.
- Pathology