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dc.contributor.authorClifton, Donald K.en_US
dc.contributor.authorMcClain, Molly E.en_US
dc.contributor.authorSteiner, Robert A.en_US
dc.contributor.authorJureus, Andersen_US
dc.contributor.authorCunningham, Matthew J.en_US
dc.date.accessioned2008-10-17T20:40:07Z
dc.date.available2008-10-17T20:40:07Z
dc.date.issued2000-07en_US
dc.identifier.citationEndocrinology. 2000 Jul;141(7):2703-6en_US
dc.identifier.urihttp://hdl.handle.net/1773/4287
dc.description.abstractGalanin-like peptide (GALP), which was recently isolated from the porcine hypothalamus, shares sequence homology with galanin and binds with high affinity to galanin receptors. To study the distribution and regulation of GALP-expressing cells in the brain, we cloned a 120 base-pair cDNA fragment of rat GALP and produced an antisense riboprobe. In situ hybridization for GALP mRNA was then performed on tissue sections throughout the forebrain of adult ovariectomized female rats. We found GALP mRNA-containing cells in the arcuate nucleus (Arc), caudal dorsomedial nucleus, median eminence and the pituitary. Because GALP mRNA in the Arc appeared to overlap with the known distribution of leptin receptor mRNA, we tested the hypothesis that GALP expression is regulated by leptin. Using in situ hybridization, we compared the number of GALP mRNA-containing cells among groups of rats that were fed ad lib or fasted for 48 h and treated with either leptin or vehicle. Fasting reduced the number of identifiable cells containing GALP mRNA in the Arc, whereas the treatment of fasted animals with leptin produced a 4-fold increase in the number of cells expressing GALP message. The presence of GALP mRNA in the hypothalamus and pituitary and its regulation by leptin suggests that GALP may have important neuroendocrine functions, including the physiological regulation of feeding, metabolism, and reproduction.en_US
dc.language.isoen_USen_US
dc.publisherEndocrine Societyen_US
dc.subjectgalanin-like peptideen_US
dc.subjectin situ hybridizationen_US
dc.subjecthypothalamusen_US
dc.subjectleptinen_US
dc.subjectraten_US
dc.subject.meshNerve Tissue Proteins, genetics, metabolismen_US
dc.subject.meshLeptin, pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshResearch Support, Non-U.S. Gov'ten_US
dc.subject.meshRNA, Messenger, metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshHypothalamus, cytology, drug effects, metabolismen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshGalanin-Like Peptideen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshFasting, metabolismen_US
dc.titleGalanin-like peptide (GALP) is a target for regulation by leptin in the hypothalamus of the raten_US
dc.typeArticleen_US


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