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Galanin-like peptide (GALP) is a target for regulation by leptin in the hypothalamus of the rat

Show simple item record Clifton, Donald K. en_US McClain, Molly E. en_US Steiner, Robert A. en_US Jureus, Anders en_US Cunningham, Matthew J. en_US 2008-10-17T20:40:07Z 2008-10-17T20:40:07Z 2000-07 en_US
dc.identifier.citation Endocrinology. 2000 Jul;141(7):2703-6 en_US
dc.description.abstract Galanin-like peptide (GALP), which was recently isolated from the porcine hypothalamus, shares sequence homology with galanin and binds with high affinity to galanin receptors. To study the distribution and regulation of GALP-expressing cells in the brain, we cloned a 120 base-pair cDNA fragment of rat GALP and produced an antisense riboprobe. In situ hybridization for GALP mRNA was then performed on tissue sections throughout the forebrain of adult ovariectomized female rats. We found GALP mRNA-containing cells in the arcuate nucleus (Arc), caudal dorsomedial nucleus, median eminence and the pituitary. Because GALP mRNA in the Arc appeared to overlap with the known distribution of leptin receptor mRNA, we tested the hypothesis that GALP expression is regulated by leptin. Using in situ hybridization, we compared the number of GALP mRNA-containing cells among groups of rats that were fed ad lib or fasted for 48 h and treated with either leptin or vehicle. Fasting reduced the number of identifiable cells containing GALP mRNA in the Arc, whereas the treatment of fasted animals with leptin produced a 4-fold increase in the number of cells expressing GALP message. The presence of GALP mRNA in the hypothalamus and pituitary and its regulation by leptin suggests that GALP may have important neuroendocrine functions, including the physiological regulation of feeding, metabolism, and reproduction. en_US
dc.language.iso en_US en_US
dc.publisher Endocrine Society en_US
dc.subject galanin-like peptide en_US
dc.subject in situ hybridization en_US
dc.subject hypothalamus en_US
dc.subject leptin en_US
dc.subject rat en_US
dc.subject.mesh Nerve Tissue Proteins, genetics, metabolism en_US
dc.subject.mesh Leptin, pharmacology en_US
dc.subject.mesh Rats en_US
dc.subject.mesh Research Support, Non-U.S. Gov't en_US
dc.subject.mesh RNA, Messenger, metabolism en_US
dc.subject.mesh Female en_US
dc.subject.mesh Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Rats, Sprague-Dawley en_US
dc.subject.mesh Hypothalamus, cytology, drug effects, metabolism en_US
dc.subject.mesh In Situ Hybridization en_US
dc.subject.mesh Galanin-Like Peptide en_US
dc.subject.mesh Tissue Distribution en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Fasting, metabolism en_US
dc.title Galanin-like peptide (GALP) is a target for regulation by leptin in the hypothalamus of the rat en_US
dc.type Article en_US

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