Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse

ResearchWorks/Manakin Repository

Search ResearchWorks


Advanced Search

Browse

My Account

Statistics

Related Information

Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse

Show full item record

Title: Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse
Author: Gottsch, Michelle L.; Clifton, Donald K.; Stoll, Elizabeth A.; Steiner, Robert A.; Eacker, Stephen M.; Braun, Robert E.; Smith, Jeremy T.; Dungan, Heather M.
Abstract: Kisspeptins are products of the Kiss1 gene, which bind to GPR54, a G protein-coupled receptor. Kisspeptins and GPR54 have been implicated in the neuroendocrine regulation of GnRH secretion. To test the hypothesis that testosterone regulates Kiss1 gene expression, we compared the expression of KiSS-1 mRNA among groups of intact, castrated, and castrated/testosterone (T)-treated male mice. In the arcuate nucleus (Arc), castration resulted in a significant increase in KiSS-1 mRNA, which was completely reversed with T replacement, whereas in the anteroventral periventricular nucleus, the results were the opposite, i.e. castration decreased and T increased KiSS-1 mRNA expression. In the Arc, the effects of T on KiSS-1 mRNA were completely mimicked by estrogen but only partially mimicked by dihydrotestosterone, a nonaromatizable androgen, suggesting that both estrogen receptor (ER) and androgen receptor (AR) play a role in T-mediated regulation of KiSS-1. Studies of the effects of T on KiSS-1 expression in mice with either a deletion of the ERalpha or a hypomorphic allele to the AR revealed that the effects of T are mediated by both ERalpha and AR pathways, which was confirmed by the presence of either ERalpha or AR coexpression in most KiSS-1 neurons in the Arc. These observations suggest that KiSS-1 neurons in the Arc, whose transcriptional activity is inhibited by T, are targets for the negative feedback regulation of GnRH secretion, whereas KiSS-1 neurons in the anteroventral periventricular nucleus, whose activity is stimulated by T, may mediate other T-dependent processes.
URI: http://hdl.handle.net/1773/4308

Files in this item

Files Size Format View
Endo_2005_Differential_Regulation_Kiss1_mRNA.pdf 656.9Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record