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Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse

Show simple item record Gottsch, Michelle L. en_US Clifton, Donald K. en_US Stoll, Elizabeth A. en_US Steiner, Robert A. en_US Eacker, Stephen M. en_US Braun, Robert E. en_US Smith, Jeremy T. en_US Dungan, Heather M. en_US 2008-10-17T20:40:29Z 2008-10-17T20:40:29Z 2005-07 en_US
dc.identifier.citation Endocrinology. 2005 Jul;146(7):2976-84. Epub 2005 Apr 14 en_US
dc.description.abstract Kisspeptins are products of the Kiss1 gene, which bind to GPR54, a G protein-coupled receptor. Kisspeptins and GPR54 have been implicated in the neuroendocrine regulation of GnRH secretion. To test the hypothesis that testosterone regulates Kiss1 gene expression, we compared the expression of KiSS-1 mRNA among groups of intact, castrated, and castrated/testosterone (T)-treated male mice. In the arcuate nucleus (Arc), castration resulted in a significant increase in KiSS-1 mRNA, which was completely reversed with T replacement, whereas in the anteroventral periventricular nucleus, the results were the opposite, i.e. castration decreased and T increased KiSS-1 mRNA expression. In the Arc, the effects of T on KiSS-1 mRNA were completely mimicked by estrogen but only partially mimicked by dihydrotestosterone, a nonaromatizable androgen, suggesting that both estrogen receptor (ER) and androgen receptor (AR) play a role in T-mediated regulation of KiSS-1. Studies of the effects of T on KiSS-1 expression in mice with either a deletion of the ERalpha or a hypomorphic allele to the AR revealed that the effects of T are mediated by both ERalpha and AR pathways, which was confirmed by the presence of either ERalpha or AR coexpression in most KiSS-1 neurons in the Arc. These observations suggest that KiSS-1 neurons in the Arc, whose transcriptional activity is inhibited by T, are targets for the negative feedback regulation of GnRH secretion, whereas KiSS-1 neurons in the anteroventral periventricular nucleus, whose activity is stimulated by T, may mediate other T-dependent processes. en_US
dc.language.iso en_US en_US
dc.publisher Endocrine Society en_US
dc.subject gene expression regulation en_US
dc.subject kisspeptin en_US
dc.subject mRNA en_US
dc.subject mice en_US
dc.subject.mesh Estrogen Receptor alpha, deficiency, genetics en_US
dc.subject.mesh Research Support, U.S. Gov't, Non-P.H.S. en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Estradiol, pharmacology en_US
dc.subject.mesh In Situ Hybridization en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Hormones, blood en_US
dc.subject.mesh Tissue Distribution en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Prosencephalon, drug effects, metabolism en_US
dc.subject.mesh Mice, Inbred C57BL en_US
dc.subject.mesh Mice, Knockout en_US
dc.subject.mesh RNA, Messenger, metabolism en_US
dc.subject.mesh Male en_US
dc.subject.mesh Brain, metabolism en_US
dc.subject.mesh Dihydrotestosterone, pharmacology en_US
dc.subject.mesh Body Weight en_US
dc.subject.mesh Orchiectomy en_US
dc.subject.mesh Testosterone, pharmacology, physiology en_US
dc.subject.mesh Research Support, N.I.H., Extramural en_US
dc.subject.mesh Receptors, Androgen, deficiency, genetics en_US
dc.subject.mesh Proteins, genetics en_US
dc.title Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse en_US
dc.type Article en_US

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