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dc.contributor.authorGottsch, Michelle L.en_US
dc.contributor.authorClifton, Donald K.en_US
dc.contributor.authorStoll, Elizabeth A.en_US
dc.contributor.authorSteiner, Robert A.en_US
dc.contributor.authorEacker, Stephen M.en_US
dc.contributor.authorBraun, Robert E.en_US
dc.contributor.authorSmith, Jeremy T.en_US
dc.contributor.authorDungan, Heather M.en_US
dc.date.accessioned2008-10-17T20:40:29Z
dc.date.available2008-10-17T20:40:29Z
dc.date.issued2005-07en_US
dc.identifier.citationEndocrinology. 2005 Jul;146(7):2976-84. Epub 2005 Apr 14en_US
dc.identifier.urihttp://hdl.handle.net/1773/4308
dc.description.abstractKisspeptins are products of the Kiss1 gene, which bind to GPR54, a G protein-coupled receptor. Kisspeptins and GPR54 have been implicated in the neuroendocrine regulation of GnRH secretion. To test the hypothesis that testosterone regulates Kiss1 gene expression, we compared the expression of KiSS-1 mRNA among groups of intact, castrated, and castrated/testosterone (T)-treated male mice. In the arcuate nucleus (Arc), castration resulted in a significant increase in KiSS-1 mRNA, which was completely reversed with T replacement, whereas in the anteroventral periventricular nucleus, the results were the opposite, i.e. castration decreased and T increased KiSS-1 mRNA expression. In the Arc, the effects of T on KiSS-1 mRNA were completely mimicked by estrogen but only partially mimicked by dihydrotestosterone, a nonaromatizable androgen, suggesting that both estrogen receptor (ER) and androgen receptor (AR) play a role in T-mediated regulation of KiSS-1. Studies of the effects of T on KiSS-1 expression in mice with either a deletion of the ERalpha or a hypomorphic allele to the AR revealed that the effects of T are mediated by both ERalpha and AR pathways, which was confirmed by the presence of either ERalpha or AR coexpression in most KiSS-1 neurons in the Arc. These observations suggest that KiSS-1 neurons in the Arc, whose transcriptional activity is inhibited by T, are targets for the negative feedback regulation of GnRH secretion, whereas KiSS-1 neurons in the anteroventral periventricular nucleus, whose activity is stimulated by T, may mediate other T-dependent processes.en_US
dc.language.isoen_USen_US
dc.publisherEndocrine Societyen_US
dc.subjectgene expression regulationen_US
dc.subjectkisspeptinen_US
dc.subjectmRNAen_US
dc.subjectmiceen_US
dc.subject.meshEstrogen Receptor alpha, deficiency, geneticsen_US
dc.subject.meshResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshEstradiol, pharmacologyen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMiceen_US
dc.subject.meshHormones, blooden_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshProsencephalon, drug effects, metabolismen_US
dc.subject.meshMice, Inbred C57BLen_US
dc.subject.meshMice, Knockouten_US
dc.subject.meshRNA, Messenger, metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshBrain, metabolismen_US
dc.subject.meshDihydrotestosterone, pharmacologyen_US
dc.subject.meshBody Weighten_US
dc.subject.meshOrchiectomyen_US
dc.subject.meshTestosterone, pharmacology, physiologyen_US
dc.subject.meshResearch Support, N.I.H., Extramuralen_US
dc.subject.meshReceptors, Androgen, deficiency, geneticsen_US
dc.subject.meshProteins, geneticsen_US
dc.titleDifferential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouseen_US
dc.typeArticleen_US


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