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A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor

Show simple item record Braun, Robert E. en_US Lee, Keesook en_US Fajardo, Mark A. en_US 2008-10-17T20:40:56Z 2008-10-17T20:40:56Z 1996-06 en_US
dc.identifier.citation Mol Cell Biol. 1996 Jun;16(6):3023-34 en_US
dc.description.abstract Translation of the mouse protamine 1 (Prm-1) mRNA is repressed for several days during male germ cell differentiation. With the hope of cloning genes that regulate the translational repression of Prm-1, we screened male germ cell cDNA expression libraries with the 3' untranslated region of the Prm-1 RNA. From this screen we obtained two independent clones that encode Prbp, a Prm-1 RNA-binding protein. Prbp contains two copies of a double-stranded-RNA-binding domain. In vitro, the protein binds to a portion of the Prm-1 3' untranslated region previously shown to be sufficient for translational repression in transgenic mice, as well as to poly(I). poly(C). Prbp protein is present in multiple forms in cytoplasmic extracts prepared from wild-type mouse testes and is absent from testes of germ cell-deficient mouse mutants, suggesting that Prbp is restricted to the germ cells of the testis. Immunocytochemical localization confirmed that Prbp is present in the cytoplasmic compartment of late-stage meiotic cells and haploid round spermatids. Recombinant Prbp protein inhibits the translation of multiple mRNAs in a wheat germ lysate, suggesting that Prbp acts to repress translation in round spermatids. While this protein lacks complete specificity for Prm-1-containing RNAs in vitro, the properties of Prbp are consistent with it acting as a general repressor of translation. en_US
dc.language.iso en_US en_US
dc.publisher American Society for Microbiology en_US
dc.subject mRNA en_US
dc.subject mouse en_US
dc.subject testis en_US
dc.subject protamine en_US
dc.subject.mesh Male en_US
dc.subject.mesh Spermatogenesis, genetics, physiology en_US
dc.subject.mesh Testis, metabolism en_US
dc.subject.mesh In Vitro en_US
dc.subject.mesh Molecular Sequence Data en_US
dc.subject.mesh Protamines, genetics en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Amino Acid Sequence en_US
dc.subject.mesh Cytoplasm, metabolism en_US
dc.subject.mesh RNA-Binding Proteins, genetics, metabolism en_US
dc.subject.mesh Protein Binding en_US
dc.subject.mesh Protein Biosynthesis en_US
dc.subject.mesh Base Sequence en_US
dc.subject.mesh Research Support, Non-U.S. Gov't en_US
dc.subject.mesh Nucleic Acid Conformation en_US
dc.subject.mesh Gene Expression Regulation, Developmental en_US
dc.subject.mesh Humans en_US
dc.subject.mesh DNA, Complementary, genetics en_US
dc.subject.mesh RNA, Messenger, chemistry, genetics, metabolism en_US
dc.subject.mesh Binding Sites, genetics en_US
dc.subject.mesh Tissue Distribution en_US
dc.subject.mesh Sequence Homology, Amino Acid en_US
dc.subject.mesh Cloning, Molecular en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Repressor Proteins, genetics, metabolism en_US
dc.title A testis cytoplasmic RNA-binding protein that has the properties of a translational repressor en_US
dc.type Article en_US

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