Show simple item record

dc.contributor.authorBraun, Robert E.en_US
dc.contributor.authorLee, Keesooken_US
dc.contributor.authorFajardo, Mark A.en_US
dc.date.accessioned2008-10-17T20:40:56Z
dc.date.available2008-10-17T20:40:56Z
dc.date.issued1996-06en_US
dc.identifier.citationMol Cell Biol. 1996 Jun;16(6):3023-34en_US
dc.identifier.urihttp://hdl.handle.net/1773/4328
dc.description.abstractTranslation of the mouse protamine 1 (Prm-1) mRNA is repressed for several days during male germ cell differentiation. With the hope of cloning genes that regulate the translational repression of Prm-1, we screened male germ cell cDNA expression libraries with the 3' untranslated region of the Prm-1 RNA. From this screen we obtained two independent clones that encode Prbp, a Prm-1 RNA-binding protein. Prbp contains two copies of a double-stranded-RNA-binding domain. In vitro, the protein binds to a portion of the Prm-1 3' untranslated region previously shown to be sufficient for translational repression in transgenic mice, as well as to poly(I). poly(C). Prbp protein is present in multiple forms in cytoplasmic extracts prepared from wild-type mouse testes and is absent from testes of germ cell-deficient mouse mutants, suggesting that Prbp is restricted to the germ cells of the testis. Immunocytochemical localization confirmed that Prbp is present in the cytoplasmic compartment of late-stage meiotic cells and haploid round spermatids. Recombinant Prbp protein inhibits the translation of multiple mRNAs in a wheat germ lysate, suggesting that Prbp acts to repress translation in round spermatids. While this protein lacks complete specificity for Prm-1-containing RNAs in vitro, the properties of Prbp are consistent with it acting as a general repressor of translation.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectmRNAen_US
dc.subjectmouseen_US
dc.subjecttestisen_US
dc.subjectprotamineen_US
dc.subject.meshMaleen_US
dc.subject.meshSpermatogenesis, genetics, physiologyen_US
dc.subject.meshTestis, metabolismen_US
dc.subject.meshIn Vitroen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProtamines, geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCytoplasm, metabolismen_US
dc.subject.meshRNA-Binding Proteins, genetics, metabolismen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshResearch Support, Non-U.S. Gov'ten_US
dc.subject.meshNucleic Acid Conformationen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshHumansen_US
dc.subject.meshDNA, Complementary, geneticsen_US
dc.subject.meshRNA, Messenger, chemistry, genetics, metabolismen_US
dc.subject.meshBinding Sites, geneticsen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshRepressor Proteins, genetics, metabolismen_US
dc.titleA testis cytoplasmic RNA-binding protein that has the properties of a translational repressoren_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record