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dc.contributor.authorSteiner, Robert A.en_US
dc.contributor.authorCunningham, Matthew J.en_US
dc.contributor.authorScarlett, Jarrad M.en_US
dc.date.accessioned2008-10-17T20:41:18Z
dc.date.available2008-10-17T20:41:18Z
dc.date.issued2002-03en_US
dc.identifier.citationEndocrinology. 2002 Mar;143(3):755-63en_US
dc.identifier.urihttp://hdl.handle.net/1773/4345
dc.description.abstractGalanin-like peptide (GALP) is a newly discovered hypothalamic neuropeptide, which is regulated by leptin and implicated in the regulation of GnRH secretion in the rodent. We searched the human genome database and determined that the human GALP gene comprises six exons, as has been shown for human galanin. We used rapid amplification of cDNA ends to clone a full-length cDNA (802 bp) of the macaque homologue of GALP and found it to be highly conserved between human and macaque at both the nucleotide (93%) and peptide (94%) levels. Mature GALP is predicted to be 60 amino acids in the macaque as in other species, and the region of GALP (9-21) that shows homology to the N-terminal 13 amino acids of galanin is perfectly conserved. We mapped the distribution of GALP mRNA in the hypothalamus and pituitary of the macaque by in situ hybridization and observed that, as in rodent species, the expression of GALP mRNA is confined to the arcuate nucleus, median eminence, and neurohypophysis. Using double-label in situ hybridization, we found that nearly all (98%) GALP mRNA-expressing cells in the arcuate nucleus also express mRNA for the long form of the leptin receptor. These findings suggest that a leptin-GALP signaling pathway exists in a primate species.en_US
dc.language.isoen_USen_US
dc.publisherEndocrine Societyen_US
dc.subjectmRNAen_US
dc.subjecthypothalamusen_US
dc.subjectpituitaryen_US
dc.subjectmacaqueen_US
dc.subjectgalanin-like peptideen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMacaca nemestrinaen_US
dc.subject.meshPituitary Gland, metabolismen_US
dc.subject.meshRNA, Messenger, biosynthesisen_US
dc.subject.meshNerve Tissue Proteins, biosynthesis, metabolismen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshGalanin-Like Peptideen_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshRNA Probesen_US
dc.subject.meshHumansen_US
dc.subject.meshHypothalamus, metabolismen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.titleCloning and distribution of galanin-like peptide mRNA in the hypothalamus and pituitary of the macaqueen_US
dc.typeArticleen_US


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