Androgen receptor gene expression in the rat central nervous system: evidence for two mRNA transcripts

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Androgen receptor gene expression in the rat central nervous system: evidence for two mRNA transcripts

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dc.contributor.author Miller, Margaret A. en_US
dc.contributor.author Dorsa, Daniel M. en_US
dc.contributor.author Bremner, William J. en_US
dc.contributor.author McLachlan, Robert I. en_US
dc.contributor.author Tempel, Bruce L. en_US
dc.date.accessioned 2008-10-17T20:41:57Z
dc.date.available 2008-10-17T20:41:57Z
dc.date.issued 1991-04 en_US
dc.identifier.citation Mol Cell Neurosci. 1991 Apr;2(2):117-122 en_US
dc.identifier.uri http://hdl.handle.net/1773/4387
dc.description.abstract Androgen receptor (AR) gene expression in the central nervous system (CNS) and peripheral tissues of male rats was examined using cDNA probes to measure AR mRNA by RNA (Northern) blot analysis and by in situ hybridization. Using a probe from the 5′ untranslated region of the rat cDNA (AR-1), a single mRNA species of approximately 11 kb was seen in Northern blots of poly(A)+ RNA from reproductive tissues, kidney, liver, and muscle. Using a probe from the 5′ end of the coding region (AR-2), in addition to the 11-kb band, a novel transcript was seen in whole brain at about 9.3 kb. In poly(A)+ RNA from dissected brain regions, the 9.3-kb transcript was predominant in the cortex, cerebellum, and brain stem, while in the hippocampus, both transcripts were expressed to a similar degree. AR mRNA levels increased two- to threefold in the prostate on Days 1 and 3 following castration but no significant change was seen in either CNS transcript in whole brain or cortex. Specific in situ hybridization of an 35S-labeled AR-2 riboprobe was observed in brain regions known to bind radiolabeled androgens. We conclude that two AR RNA species exist in the adult male rat which differ in their 5′ untranslated region and that the relative proportion of the two species varies between brain regions. In contrast to observations in the prostate, AR gene expression in the cerebral cortex is not regulated in the short term by androgen withdrawal. en_US
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject spermatogenesis en_US
dc.subject testosterone en_US
dc.subject male contraception en_US
dc.subject andrology en_US
dc.subject gonadotropins en_US
dc.subject colchicine en_US
dc.subject 5-alpha reductase inhibitors en_US
dc.subject klinefelter's syndrome en_US
dc.subject reifenstein's syndrome en_US
dc.title Androgen receptor gene expression in the rat central nervous system: evidence for two mRNA transcripts en_US
dc.type Article en_US


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