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dc.contributor.authorClifton, Donald K.en_US
dc.contributor.authorSteiner, Robert A.en_US
dc.contributor.authorHohmann, John G.en_US
dc.contributor.authorCheung, Clement C.en_US
dc.date.accessioned2008-10-17T20:42:01Z
dc.date.available2008-10-17T20:42:01Z
dc.date.issued2001en_US
dc.identifier.citationNeuroscience. 2001;103(2):423-32en_US
dc.identifier.urihttp://hdl.handle.net/1773/4392
dc.description.abstractGalanin is widely distributed throughout the mammalian brain and has been implicated in the regulation of food intake, metabolism and reproduction-functions that are also thought to be under the control of leptin. To investigate the possible role of galanin in mediating the physiological effects of leptin in the mouse, we had three experimental objectives: first, to map the distribution of galanin messenger RNA-expressing cells in the brain of the mouse; second, to assess the effects of leptin on galanin gene expression in areas of the brain thought to be involved in the regulation of body weight and reproduction; and third, to determine whether galanin neurons in these regions express leptin receptor messenger RNA. We found the pattern of galanin messenger RNA expression in the mouse brain to be similar, but not identical, to that in the rat. Leptin treatment (2microg/g for six days) significantly reduced cellular levels of galanin messenger RNA in the hypothalamic periventricular nucleus of leptin-deficient obese (ob/ob) mice (P<0.01) by approximately 30%; however, leptin did not appear to influence the expression of galanin in the arcuate or dorsomedial nucleus of the hypothalamus. Galanin-producing neurons in the arcuate, dorsomedial and periventricular nuclei did not appear to express leptin receptor messenger RNA (P>0.05). These results demonstrate that galanin distribution patterns in the mouse brain are comparable to other species and, yet, possess unique features. In addition, galanin-expressing neurons in the hypothalamic periventricular nucleus are targets for regulation by leptin; however, the effect of leptin on galanin gene expression is likely to be mediated indirectly, perhaps through either proopiomelanocortin- or neuropeptide Y-expressing cells in the hypothalamus.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.subjectneuropeptideen_US
dc.subjectgene regulationen_US
dc.subjectneuroanatomyen_US
dc.subjectleptin receptoren_US
dc.subjectin situ hybridizationen_US
dc.subject.meshDorsomedial Hypothalamic Nucleus, drug effects, physiologyen_US
dc.subject.meshGene Expression Regulation, drug effects, physiologyen_US
dc.subject.meshResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshCarrier Proteins, physiologyen_US
dc.subject.meshLeptin, genetics, pharmacologyen_US
dc.subject.meshGalanin, geneticsen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshReceptors, Cell Surfaceen_US
dc.subject.meshMaleen_US
dc.subject.meshParaventricular Hypothalamic Nucleus, drug effects, physiologyen_US
dc.subject.meshBrain Chemistry, geneticsen_US
dc.subject.meshMice, Inbred C57BLen_US
dc.subject.meshMice, Obeseen_US
dc.subject.meshArcuate Nucleus, drug effects, physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshRNA, Messenger, analysisen_US
dc.subject.meshObesity, genetics, physiopathologyen_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshMiceen_US
dc.titleDistribution of galanin messenger RNA-expressing cells in murine brain and their regulation by leptin in regions of the hypothalamusen_US
dc.typeArticleen_US


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