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dc.contributor.advisorParichy, David
dc.contributor.advisorRaible, David
dc.contributor.authorLewis, Victor Maxwell
dc.date.accessioned2019-08-14T22:29:46Z
dc.date.available2019-08-14T22:29:46Z
dc.date.submitted2019
dc.identifier.otherLewis_washington_0250E_20439.pdf
dc.identifier.urihttp://hdl.handle.net/1773/44062
dc.descriptionThesis (Ph.D.)--University of Washington, 2019
dc.description.abstractUnderstanding genetic and cellular bases of adult form remains a fundamental goal at the intersection of developmental and evolutionary biology. The skin pigment cells of vertebrates, derived from embryonic neural crest, are a useful system for elucidating mechanisms of fate specification, pattern formation, and how particular phenotypes impact organismal behavior and ecology. In a survey of Danio fishes, including the zebrafish Danio rerio, we identified two populations of white pigment cells—leucophores—one of which arises by transdifferentiation of adult melanophores and another of which develops from a yellow–orange xanthophore or xanthophore-like progenitor. Single-cell transcriptomic, mutational, chemical, and ultrastructural analyses of zebrafish leucophores revealed cell-type–specific chemical compositions, organelle configurations, and genetic requirements. At the organismal level, we identified distinct physiological responses of leucophores during environmental background matching, and we showed that leucophore complement influences behavior. Together, our studies reveal independently arisen pigment cell types and mechanisms of fate acquisition in zebrafish and illustrate how concerted analyses across hierarchical levels can provide insights into phenotypes and their evolution.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.rightsnone
dc.subjectevolution
dc.subjectleucophore
dc.subjectpigment cell
dc.subjecttransdifferentiation
dc.subjectzebrafish
dc.subjectDevelopmental biology
dc.subjectEvolution & development
dc.subject.otherBiology
dc.titleFate plasticity and reprogramming in genetically distinct populations of Danio leucophores
dc.typeThesis
dc.embargo.termsOpen Access


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