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dc.contributor.authorNadler, Jessica J.en_US
dc.contributor.authorBraun, Robert E.en_US
dc.date.accessioned2008-10-17T20:42:20Z
dc.date.available2008-10-17T20:42:20Z
dc.date.issued2000-07en_US
dc.identifier.citationGenesis. 2000 Jul;27(3):117-23en_US
dc.identifier.urihttp://hdl.handle.net/1773/4413
dc.description.abstractFanconi anemia is a polygenic trait hypothesized to be a DNA damage repair disease. We show that all three Fanconi anemia loci that have been cloned are expressed in the embryonic gonad during the period of primordial germ cell proliferation. Mice mutant for the Fanconi anemia complementation group C locus (Fancc) have reduced germ cell numbers as early as embryonic day E12.5, suggesting the Fancc protein functions prior to meiosis in both sexes. Depletion in the mutant occurs at a time when all three loci would be expressed in a wild-type gonad, implying a function in the early germline. Determination of the mitotic index of primordial germ cells by BrdU incorporation shows that germ cells in Fancc(-/-) mice proliferate significantly more slowly than littermate controls. This study demonstrates Fancc is required for mitotic proliferation of primordial germ cells.en_US
dc.language.isoen_USen_US
dc.publisherWiley-Lissen_US
dc.subjectmiceen_US
dc.subjectgene expressionen_US
dc.subjectprimordial germ cellsen_US
dc.subjectFanconi anemiaen_US
dc.subject.meshMeiosisen_US
dc.subject.meshTestis, embryologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMice, Inbred C57BLen_US
dc.subject.meshMitotic Indexen_US
dc.subject.meshMiceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Cycle Proteinsen_US
dc.subject.meshFanconi Anemia Complementation Group C Proteinen_US
dc.subject.meshPregnancyen_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshOvary, embryologyen_US
dc.subject.meshDNA-Binding Proteinsen_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshMice, Mutant Strainsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshFanconi Anemia Complementation Group Proteinsen_US
dc.subject.meshOvum, cytology, physiologyen_US
dc.subject.meshEmbryonic and Fetal Development, geneticsen_US
dc.subject.meshSpermatozoa, cytology, physiologyen_US
dc.subject.meshFanconi Anemia, geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshProteins, genetics, metabolismen_US
dc.titleFanconi anemia complementation group C is required for proliferation of murine primordial germ cellsen_US
dc.typeArticleen_US


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