Show simple item record

dc.contributor.authorHohmann, John G.en_US
dc.contributor.authorJureus, Andersen_US
dc.contributor.authorTeklemichael, Dawit N.en_US
dc.contributor.authorMatsumoto, Alvin M.en_US
dc.contributor.authorClifton, Donald K.en_US
dc.contributor.authorSteiner, Robert A.en_US
dc.date.accessioned2008-10-17T20:42:33Z
dc.date.available2008-10-17T20:42:33Z
dc.date.issued2003en_US
dc.identifier.citationNeuroscience. 2003;117(1):105-17en_US
dc.identifier.urihttp://hdl.handle.net/1773/4426
dc.description.abstractTo learn more about molecular alterations in the brain that occur as a consequence of either the chronic excess or absence of peptide neurotransmitters, we examined the impact of genetically manipulating the neuropeptide galanin on the expression of one of its cognate receptors, galanin receptor 1. First, we examined the distribution of galanin receptor 1 messenger RNA in the mouse forebrain, and found it to be abundantly expressed in many brain regions, including in numerous hypothalamic and other forebrain regions associated with neuroendocrine function. The distribution of galanin receptor 1 messenger RNA in the mouse was similar to previous reports in the rat, with additional expression noted in the caudate putamen and in several midbrain regions. Next, using quantitative in situ hybridization, we measured cellular levels of galanin receptor 1 messenger RNA in the brains of mice that either overexpress galanin (galanin transgenic) or lack a functional galanin gene (galanin knockout). We report that relative to wild-type controls, the expression of galanin receptor 1 messenger RNA was increased in discrete areas of the brain in galanin-transgenic mice, but that depletion of galanin/noradrenergic innervation to the hypothalamus with the neurotoxin 6-hydroxydopamine did not alter levels of galanin receptor 1 messenger RNA. We also report that levels of galanin receptor 1 messenger RNA were not different between galanin-knockout and wild-type mice. These results suggest that compensatory adjustments in the expression of cognate receptors represent one mechanism by which the developing nervous system attempts to maintain homeostasis in response to overexpression of a peptidergic transmitter. However, the lack of significant changes in galanin receptor 1 messenger RNA in galanin-knockout mice suggests that developmentally programmed levels of receptor expression are maintained even in the complete absence of ligand.en_US
dc.language.isoen_USen_US
dc.publisherEndocrine Societyen_US
dc.subjectgalaninen_US
dc.subjecttransgenicen_US
dc.subjectreceptoren_US
dc.subjectknockouten_US
dc.subjecthypothalamusen_US
dc.subjectmouseen_US
dc.subject.meshMice, Knockouten_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshMaleen_US
dc.subject.meshResearch Support, Non-U.S. Gov'ten_US
dc.subject.meshResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshComparative Studyen_US
dc.subject.meshProsencephalon, chemistry, metabolismen_US
dc.subject.meshReceptors, Galaninen_US
dc.subject.meshMiceen_US
dc.subject.meshReceptors, Neuropeptide, analysis, biosynthesis, deficiency, geneticsen_US
dc.subject.meshRNA, Messenger, analysis, biosynthesisen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshMice, Inbred C57BLen_US
dc.titleDistribution and regulation of galanin receptor 1 messenger RNA in the forebrain of wild type and galanin-transgenic miceen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record