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Absence of germline infection in male mice following intraventricular injection of adenovirus

Show simple item record Braun, Robert E. en_US Roth, David A. en_US Novak, Patricia L. en_US Ferrell, Carolyn en_US Friedman, Jeffrey en_US McCaman, Michael en_US Roth, David M. en_US Engler, Robert en_US Peters, Antoine H. F. M. en_US Drumm, Jeff en_US 2008-10-17T20:42:57Z 2008-10-17T20:42:57Z 2001-12 en_US
dc.identifier.citation Mol Ther. 2001 Dec;4(6):603-13 en_US
dc.description.abstract The possibility of inadvertent exposure of gonadal tissue to gene therapy vectors has raised safety concerns about germline infection. We show here that the receptor for coxsackie B viruses and adenoviruses 2 and 5 (CXADR) is expressed in mouse germ cells, suggesting the possibility that these viruses could infect germ cells. To directly assess the risk of germline infection in vivo, we injected an adenovirus carrying the germ-cell-specific protamine promoter fused to the bacterial lacZ reporter gene into the left ventricular cavity of mice and then monitored expression of the reporter gene in germ cells. To differentiate between infection of stem cells and differentiating spermatogenic cells, we analyzed expression of the reporter cassette at different times after viral delivery. Under all conditions tested, mice did not express the Escherichia coli beta-galactosidase protein in developing spermatids or in mature epididymal spermatozoa. Primary germ cells cultured in vitro were also refractory to adenoviral infection. Our data suggest that the chance of vertical germline transmission and insertional mutagenesis is highly unlikely following intracoronary adenoviral delivery. en_US
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject germline infection en_US
dc.subject sperm en_US
dc.subject protamine en_US
dc.subject testis en_US
dc.subject gene therapy en_US
dc.subject adenovirus en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Receptors, Virus, metabolism en_US
dc.subject.mesh Spermatozoa, metabolism, virology en_US
dc.subject.mesh Adenoviridae, physiology en_US
dc.subject.mesh DNA Primers, chemistry en_US
dc.subject.mesh Gene Therapy, methods en_US
dc.subject.mesh Cerebral Ventricles, virology en_US
dc.subject.mesh beta-Galactosidase, metabolism en_US
dc.subject.mesh Lac Operon en_US
dc.subject.mesh Gene Transfer Techniques en_US
dc.subject.mesh Humans en_US
dc.subject.mesh Membrane Proteins, genetics en_US
dc.subject.mesh Fluorescent Antibody Technique, Indirect en_US
dc.subject.mesh Injections, Intraventricular en_US
dc.subject.mesh Testis, metabolism, virology en_US
dc.subject.mesh Polymerase Chain Reaction en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Male en_US
dc.title Absence of germline infection in male mice following intraventricular injection of adenovirus en_US
dc.type Article en_US

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