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dc.contributor.authorBraun, Robert E.en_US
dc.contributor.authorRoth, David A.en_US
dc.contributor.authorNovak, Patricia L.en_US
dc.contributor.authorFerrell, Carolynen_US
dc.contributor.authorFriedman, Jeffreyen_US
dc.contributor.authorMcCaman, Michaelen_US
dc.contributor.authorRoth, David M.en_US
dc.contributor.authorEngler, Roberten_US
dc.contributor.authorPeters, Antoine H. F. M.en_US
dc.contributor.authorDrumm, Jeffen_US
dc.date.accessioned2008-10-17T20:42:57Z
dc.date.available2008-10-17T20:42:57Z
dc.date.issued2001-12en_US
dc.identifier.citationMol Ther. 2001 Dec;4(6):603-13en_US
dc.identifier.urihttp://hdl.handle.net/1773/4449
dc.description.abstractThe possibility of inadvertent exposure of gonadal tissue to gene therapy vectors has raised safety concerns about germline infection. We show here that the receptor for coxsackie B viruses and adenoviruses 2 and 5 (CXADR) is expressed in mouse germ cells, suggesting the possibility that these viruses could infect germ cells. To directly assess the risk of germline infection in vivo, we injected an adenovirus carrying the germ-cell-specific protamine promoter fused to the bacterial lacZ reporter gene into the left ventricular cavity of mice and then monitored expression of the reporter gene in germ cells. To differentiate between infection of stem cells and differentiating spermatogenic cells, we analyzed expression of the reporter cassette at different times after viral delivery. Under all conditions tested, mice did not express the Escherichia coli beta-galactosidase protein in developing spermatids or in mature epididymal spermatozoa. Primary germ cells cultured in vitro were also refractory to adenoviral infection. Our data suggest that the chance of vertical germline transmission and insertional mutagenesis is highly unlikely following intracoronary adenoviral delivery.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.subjectgermline infectionen_US
dc.subjectspermen_US
dc.subjectprotamineen_US
dc.subjecttestisen_US
dc.subjectgene therapyen_US
dc.subjectadenovirusen_US
dc.subject.meshAnimalsen_US
dc.subject.meshReceptors, Virus, metabolismen_US
dc.subject.meshSpermatozoa, metabolism, virologyen_US
dc.subject.meshAdenoviridae, physiologyen_US
dc.subject.meshDNA Primers, chemistryen_US
dc.subject.meshGene Therapy, methodsen_US
dc.subject.meshCerebral Ventricles, virologyen_US
dc.subject.meshbeta-Galactosidase, metabolismen_US
dc.subject.meshLac Operonen_US
dc.subject.meshGene Transfer Techniquesen_US
dc.subject.meshHumansen_US
dc.subject.meshMembrane Proteins, geneticsen_US
dc.subject.meshFluorescent Antibody Technique, Indirecten_US
dc.subject.meshInjections, Intraventricularen_US
dc.subject.meshTestis, metabolism, virologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshMiceen_US
dc.subject.meshMaleen_US
dc.titleAbsence of germline infection in male mice following intraventricular injection of adenovirusen_US
dc.typeArticleen_US


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