Show simple item record

dc.contributor.authorDisteche, Christineen_US
dc.contributor.authorEdelhoff, Susanneen_US
dc.contributor.authorBraun, Robert E.en_US
dc.contributor.authorBuass, F. Williamen_US
dc.contributor.authorLee, Keesooken_US
dc.date.accessioned2008-10-17T20:43:04Z
dc.date.available2008-10-17T20:43:04Z
dc.date.issued1999-05en_US
dc.identifier.citationMamm Genome. 1999 May;10(5):451-6en_US
dc.identifier.urihttp://hdl.handle.net/1773/4456
dc.description.abstractIn a screen for RNA-binding proteins expressed during murine spermatogenesis, we have identified a cDNA that encodes a protein of 911 amino acids that contains two copies of the double-stranded RNA-binding motif and has 80% identity with human Interleukin Enhancer Binding Factor 3 (ILF3). Linkage and cytogenetic analyses localized the Ilf3 cDNA to a portion of mouse Chr 9, which shows conserved synteny with a region of human Chr 19 where the human ILF3 gene had been previously localized, supporting that we had cloned the murine homolog of ILF3. Northern analysis indicated the Ilf3 gene is ubiquitously expressed in mouse adult tissues with high levels of expression in the brain, thymus, testis, and ovary. Polyclonal antibodies detected multiple protein species in a subset of the tissues expressing Ilf3 RNA. Immunoreactive species are present at high levels in the thymus, testis, ovary, and the spleen to a lesser extent. The high degree of sequence similarity between the mouse ILF3 protein and other dsRNA binding motif-containing proteins suggests a role in RNA metabolism, while the differential expression indicates the mouse ILF3 protein predominantly functions in tissues containing developing lymphocyte and germ cells.en_US
dc.language.isoen_USen_US
dc.publisherSpringer-Verlagen_US
dc.subjectcloningen_US
dc.subjectmouseen_US
dc.subjectRNAen_US
dc.subjectILF3en_US
dc.subject.meshHumansen_US
dc.subject.meshDNA-Binding Proteins, geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNuclear Factor 90 Proteinsen_US
dc.subject.meshMiceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshRNA-Binding Proteins, geneticsen_US
dc.subject.mesh3' Untranslated Regionsen_US
dc.subject.meshTranscription Factors, geneticsen_US
dc.subject.meshNFATC Transcription Factorsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA, Complementaryen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshResearch Support, Non-U.S. Gov'ten_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titleCloning and characterization of the mouse interleukin enhancer binding factor 3 (Ilf3) homolog in a screen for RNA binding proteinsen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record