Regulation of hypothalamic proopiomelanocortin mRNA by leptin in ob/ob mice

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Regulation of hypothalamic proopiomelanocortin mRNA by leptin in ob/ob mice

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dc.contributor.author Steiner, Robert A. en_US
dc.contributor.author Clifton, Donald K. en_US
dc.contributor.author Thornton, Janice E. en_US
dc.contributor.author Cheung, Clement C. en_US
dc.date.accessioned 2008-10-17T20:43:17Z
dc.date.available 2008-10-17T20:43:17Z
dc.date.issued 1997-11 en_US
dc.identifier.citation Endocrinology. 1997 Nov;138(11):5063-6 en_US
dc.identifier.uri http://hdl.handle.net/1773/4466
dc.description.abstract The hormone leptin acts on the brain to regulate feeding, metabolism, and reproduction; however, its cellular targets and molecular mechanisms of action remain to be fully elucidated. The melanocortins, which are derived from the precursor proopiomelanocortin (POMC), are also implicated in the physiological regulation of body weight. POMC-containing neurons express the leptin receptor, and thus it is conceivable that the POMC gene itself may be part of the signaling pathway involved in leptin's action on the brain. Using in situ hybridization and computerized image analysis, we tested the hypothesis that the POMC gene is a target for regulation by leptin by comparing cellular levels of POMC mRNA in the hypothalamus among groups of leptin-deficient (ob/ob) mice, leptin-treated ob/ob mice, and wild-type controls. POMC mRNA levels were significantly reduced throughout the arcuate nucleus in vehicle-treated ob/ob mice relative to wild-type controls, whereas POMC mRNA levels in leptin-treated ob/ob mice were indistinguishable from wild-type controls. These observations suggest that one or more products of POMC serve as an integrative link between leptin and the central mechanisms governing body weight regulation and reproduction. en_US
dc.language.iso en_US en_US
dc.publisher Endocrine Society en_US
dc.subject leptin receptor en_US
dc.subject POMC mRNA en_US
dc.subject in situ hybridization en_US
dc.subject body weight en_US
dc.subject.mesh Obesity, genetics, metabolism, pathology en_US
dc.subject.mesh Neurons, metabolism, pathology en_US
dc.subject.mesh Proteins, genetics, metabolism, pharmacology en_US
dc.subject.mesh Research Support, U.S. Gov't, P.H.S. en_US
dc.subject.mesh Pro-Opiomelanocortin, genetics en_US
dc.subject.mesh Animals en_US
dc.subject.mesh Leptin en_US
dc.subject.mesh Cell Count en_US
dc.subject.mesh RNA, Messenger, metabolism en_US
dc.subject.mesh Mice en_US
dc.subject.mesh Mice, Mutant Strains, genetics en_US
dc.subject.mesh Male en_US
dc.subject.mesh Eating, drug effects en_US
dc.subject.mesh Hypothalamus, metabolism, pathology en_US
dc.title Regulation of hypothalamic proopiomelanocortin mRNA by leptin in ob/ob mice en_US
dc.type Article en_US


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