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dc.contributor.authorJohnson, Laura L.en_US
dc.contributor.authorClifton, Donald K.en_US
dc.contributor.authorLi, Dorothyen_US
dc.contributor.authorSteiner, Robert A.en_US
dc.contributor.authorTeklemichael, Dawit N.en_US
dc.contributor.authorKrasnow, Stephanie M.en_US
dc.contributor.authorJureus, Andersen_US
dc.contributor.authorCunningham, Matthew J.en_US
dc.date.accessioned2008-10-17T20:44:08Z
dc.date.available2008-10-17T20:44:08Z
dc.date.issued2001-12en_US
dc.identifier.citationEndocrinology. 2001 Dec;142(12):5140-4en_US
dc.identifier.urihttp://hdl.handle.net/1773/4504
dc.description.abstractGalanin-like peptide (GALP) is a newly discovered molecule whose expression in the brain is confined to the arcuate nucleus and median eminence. In the rat, cellular levels of GALP mRNA are reduced by fasting and reversed by peripheral administration of leptin. The purpose of this investigation was 1) to clone and map the distribution of GALP mRNA in the brain of the mouse; 2) to compare the pattern and magnitude of GALP mRNA expression in the leptin-deficient obese (ob/ob) mouse with that of wild-type controls; and 3) to examine the effects of leptin delivered into the brain on the expression of GALP mRNA in the ob/ob mouse. We report the sequence of a mouse GALP cDNA and show that GALP mRNA is expressed in the arcuate nucleus, median eminence, infundibular stalk, and the neurohypophysis of this species. The expression of GALP mRNA in the brain was markedly reduced in the ob/ob mice, compared with wild-type animals. Intracerebroventricular infusion of leptin to ob/ob mice increased both the number of GALP mRNA-expressing neurons and their content of GALP mRNA, compared with vehicle-treated controls. These observations demonstrate that GALP mRNA is induced by leptin through a direct action on the brain.en_US
dc.language.isoen_USen_US
dc.publisherEndocrine Societyen_US
dc.subjectGALPen_US
dc.subjectleptinen_US
dc.subject.meshMaleen_US
dc.subject.meshLeptin, deficiencyen_US
dc.subject.meshMice, Inbred C57BLen_US
dc.subject.meshObesity, geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshResearch Support, U.S. Gov't, Non-P.H.S.en_US
dc.subject.meshRNA, Messenger, metabolismen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshNerve Tissue Proteins, genetics, metabolismen_US
dc.subject.meshGalanin-Like Peptideen_US
dc.subject.meshHypothalamus, metabolismen_US
dc.subject.meshDNA, Complementary, geneticsen_US
dc.subject.meshResearch Support, Non-U.S. Gov'ten_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain, metabolismen_US
dc.subject.meshResearch Support, U.S. Gov't, P.H.S.en_US
dc.titleDistribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouseen_US
dc.typeArticleen_US


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