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dc.contributor.advisorVaughan, Joshua
dc.contributor.authorHoward, Marco d
dc.date.accessioned2020-02-04T19:25:06Z
dc.date.available2020-02-04T19:25:06Z
dc.date.submitted2019
dc.identifier.otherHoward_washington_0250E_21003.pdf
dc.identifier.urihttp://hdl.handle.net/1773/45139
dc.descriptionThesis (Ph.D.)--University of Washington, 2019
dc.description.abstractSuper-Resolution microscopy has transformed our ability to image biological specimens at the nanoscale with high contrast and molecular specificity. However, acquiring 3-dimensional images over large volumes remains a challenge because the imaging process causes fluorophores outside the focal volume to photobleach before they can be imaged. Additionally, acquiring highly multiplexed images is challenging due to a lack of spectrally distinct fluorophores which possess the required photophysical properties for super-resolution imaging. Here I present my work which specifically addresses these issues. In chapter 2 I first discuss the importance of sample preparation for super-resolution imaging. In chapter 3 I introduce a novel labeling scheme called probe-refresh STORM (prSTORM) which enables practitioners obtain multiplexed, extended-depth super-resolution images with a single dye, and in chapter 4 I show the importance of hardware for super-resolution microscopy
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.rightsCC BY
dc.subjectDNA-Barcode
dc.subjectImaging
dc.subjectImmunofluorescence
dc.subjectMicroscopy
dc.subjectNanoscopy
dc.subjectSuper-Resolution
dc.subjectBioengineering
dc.subject.otherChemistry
dc.titleA New Approach to 3-Dimensional Super-Resolution Microscopy
dc.typeThesis
dc.embargo.termsOpen Access


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