Browsing Dissertations and Theses by Subject "Ubiquitin"
Now showing items 1-7 of 7
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Development of auxiliary-mediated protein semisynthesis methods toward the study of histone SUMOylation
Eukaryotic DNA is packaged into chromatin, which consists of a fundamental repeating unit, the nucleosome, and its associated proteins. Nucleosomes are made up of histone protein octamers, containing two each of histones ... -
HSP70 Isoforms Ssa1 and Ssa2 are Differentially Required in San1 Mediated Degradation
Misfolded proteins have the propensity to aggregate and become toxic to the cell if they accumulate. To counter this detrimental burden, the eukaryotic cell has developed a variety of protein quality control (PQC) mechanisms ... -
Investigating the Effect of Histone H4 Sumoylation on Chromatin Structure and Function
The packaging of DNA within the nucleus of a eukaryotic organism is facilitated by the formation of a nucleoprotein complex known as chromatin. There are various DNA binding proteins that play an important part in this ... -
Regulation of centromeric histone localization in budding yeast
The exclusive localization of the histone H3 variant CENP-A to centromeres is essential for accurate chromosome segregation. Ubiquitin-mediated proteolysis helps to ensure that CENP-A does not mislocalize to euchromatin, ... -
Regulation of Substrate Degradation and Cancer Metabolism by the Fbw7 Ubiquitin Ligase
Fbw7 is the substrate binding subunit of an SCF E3 ubiquitin ligase, which catalyzes the polyubiquitylation of substrates, initiating their recognition and degradation by the proteasome. The affinity of Fbw7 for its ... -
Structural Studies of RNF146-Mediated PARylation-Dependent Ubiquitylation
The ubiquitylation of proteins is involved in nearly every cellular process. Hence, precise control of the ligases that facilitate the attachment ubiquitin (Ub) to target substrates is of the utmost importance to eukaryotic ... -
Ubx cofactors are required in San1-dependent nuclear protein quality control
Accumulation of misfolded proteins in the cell are linked to many human diseases. To ensure optimal cellular physiology, the cell has compartmentalized the protein quality control system (PQC). As a compartment, the nucleus ...