Browsing Bioengineering by Title
Now showing items 68-87 of 356
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De novo design of protein binders as functional therapeutics
De novo design of protein binding proteins (minibinders) with target structure information alone remains a grand challenge. A general computational design framework includes (1) generation of binder backbones, (2) sequence ... -
Decoding Coordinated Hand Movement in Human Primary Motor Cortex Using High Resolution Electrocorticography
(2014-02-24)Electrophysiology studies of the brain allow us to study the dynamics of the cortex as subjects perform tasks and experiment. Of particular interest in human studies is the activity that occurs in motor related areas of ... -
Deep Learning and Cloud Science for the Engineering of Biological Molecules and Systems
Solving many of the greatest challenges facing our industrial landscape and therapeutic de- velopment will require innovations in both computational and experimental biology alike. Deep learning has demonstrated it capacity ... -
Defining murine cardiac fibroblast cellular states via fibroblast-vascular crosstalk and extracellular matrix remodeling
The overarching focus of this thesis is to gain insight into novel categories of cardiac fibroblast cellular state by probing the biological underpinnings of how structure dictates function. Cardiac fibroblasts are responsible ... -
Design and Characterization of Integrin α4β1-Specific DNA Aptamer Conjugates for Cancer Targeting Applications
Cancer can exert a burdensome toll on all in its grasp, causing physical as well as psychological distress. Therapies currently in use to treat cancer either carry a variety of unpleasant side-effects or are prohibitively ... -
Design and Construction of a Sensor to Monitor Blood Parameters During Resuscitation
When performed by professional medical staff, cardiopulimary resuscitation (CPR) is successful in resuscitating patients in less than 45% of cases. Even in cases where patients survive, brain damage due to partially hypoxic ... -
Design and Construction of His-tagged Protein Purification Workflow in Aquarium
Protein purification is a long process starting from cloning, protein induction, extraction, isolation to protein verification. Carrying out this experiment can be very time-consuming and pricey, especially when some ... -
Design and development of surface plasmon resonance imaging microfluidic assays
(2007)This dissertation focuses on the design and development of SPR-imaging microfluidic assays to quantify nanomolar concentrations of small molecules (cortisol and phenytoin) in saliva for a point-of-care medical diagnostic. ... -
Design and Evaluation of an In Vivo, Repeatable, and Real Time Reporter for Myofibroblast Cell Fate as a Surrogate for Cardiac Fibrotic Remodeling
Heart disease is the leading cause of death in the United States accounting for 1 in every 4 deaths [“Heart Disease Facts”]. More than half of heart disease deaths are attributed to coronary heart disease which often leads ... -
Design of Novel Repeat Proteins Containing Beta Strands
Repeat protein design is a natural direction for protein bioengineering thanks to inherent adaptability, geometric properties, and facile extensibility. Natural systems use repeat proteins as materials scaffolds, sensors, ... -
Designing the diffusion immunoassay (DIA): how properties of the analyte affect DIA performance
(2007)The diffusion immunoassay (DIA) is a novel assay technique based on the difference in diffusivity of an analyte molecule when free in solution, versus when it is bound to a cognate antibody. It is uniquely enabled by the ... -
Determination of alpha-sheet secondary structure as an early biomarker in the pathogenesis of Alzheimer's disease
Alzheimer’s disease (AD) is one of over 50 amyloid diseases discovered to-date and is now the sixth leading cause of death in the United States, affecting over 5.8 million Americans and projected to cost upwards of $1.1 ... -
Determining the feasibility of assessing the adequacy and immunophenotype of human breast core needle biopsies using molecular and H&E fluorescence imaging via deep-UV optical microscopy
Histopathology techniques have not advanced with related technological innovations made in the last few decades. As a result, they are needlessly time consuming, labor intensive, and are not adapted for core needle biopsies ... -
Developing a FRET tension sensor-compatible system for analyzing cardiomyocyte tension alongside applied strain
Cardiomyopathy refers to heart diseases in which myocardial tissue has undergone structural changes, often resulting in systolic dysfunction. Restructuring of the heart is influenced by mechanical forces and these organ-level ... -
Developing a library of recognition proteins using FimH as a protein scaffold
(2013-11-14)High specificity recognition proteins, like antibodies, are important biological tools with many applications, including diagnostics, therapeutics, and imaging. However, there are many challenges with antibody recognition ... -
Developing an Inducible Osteoclast System as a Cell Therapy for Ectopic Calcification
Ectopic calcification (EC) is a complex disease in which mineral develops in abnormal locations. It can develop in a variety of locations throughout the body, including the vasculature, heart valves, and in orthopedic ... -
Developing and Validating Novel Genetically Encoded Sensors in Animal Behavioral Models to Investigate KOR-Dynorphin Interactions
Deaths from the opioid epidemic have been increasing steadily in recent years and have prompted many studies investigating various neural circuits related to addiction. Previous work has shown that both κ-opioid receptor ... -
Development and Application of Augmented Smartphone-enabled Imaging in Mobile Health
Mobile health techniques aim to deliver healthcare remotely and wirelessly using surging computing power and mobile connectivity. The commercial smartphone is naturally becoming a popular partner device due to its ... -
Development and Application of Multifunctional Optical Coherence Tomography
Microcirculation refers to the functions of capillaries and the neighboring lymphatic vessels. It plays a vital role in the pathophysiology of disorders in many clinical areas including cardiology, dermatology, neurology ... -
Development and Application of Multifunctional Optical Coherence Tomography
Microcirculation refers to the functions of capillaries and the neighboring lymphatic vessels. It plays a vital role in the pathophysiology of disorders in many clinical areas including cardiology, dermatology, neurology ...