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Quantification of DNA content using 3D Optical Projection Tomographic Microscopy for Early Cancer Diagnosis
Aneuploidy, an accepted biomarker for malignancy, is typically assessed by flow (FCM) and image cytometry (ICM). We used optical projection tomographic microscopy (OPTM) for assessing cellular DNA content using different absorption and fluorescence stains. OPTM combines some attributes of both FCM and ICM and provides ...