Browsing Biological chemistry by Title
Now showing items 67-86 of 122
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Mechanism of SspH1: A Bacterial Effector Ubiquitin Ligase Hijacks the Eukaryotic Ubiquitylation Pathway
Bacterial effector proteins promote the pathogenicity of bacteria by interacting with host cell proteins and modulating signaling pathways in the host cell. Some bacterial effectors hijack the eukaryotic ubiquitylation ... -
Metabolic and Epigenetic Regulation in Development and in Embryonic Diapause
Embryonic diapause is a state of dormancy that interrupts the normally tight connection between developmental stage and time. Regulation of this dormant state, however, is poorly understood. To better understand the processes ... -
Metabolic function in the ocular ecosystem relies on interactions between the retina and retinal pigment epithelium and remains robust in aging
The vertebrate eye is a unique ecosystem comprised of a myriad of different cells that all function in concert to facilitate vision. The electrical signal in the eye that is transmitted to the brain begins with the retina, ... -
Microtubule attachment and regulation mechanisms of the budding yeast kinetochore
Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the budding yeast kinetochore. ... -
Mitofusin Disease Variant Provides Insight into Mitochondrial Tethering and Fusion
Mitochondria are dynamic organelles that produce the energy we need to move, grow, and think. Every cell, from muscles in the heart to neurons in the brain, has a unique and ever-changing requirement for energy. In order ... -
Modulation of Human Small Heat Shock Proteins via Changes in Quasi-ordered Networks of Interactions
Small heat shock proteins (sHSPs) are molecular chaperones found in all domains of life that directly interact with client proteins to maintain them in a soluble and functional state. sHSPs are among the earliest responders ... -
Molecular Architecture and Regulation of the Budding Yeast Kinetochore-Microtubule Interface
Equal partitioning of replicated chromosomes during mitosis is vital. The genetic information stored in chromosomes provide the template for cells to produce RNA and protein molecules, which carry out cellular functions. ... -
Molecular Insights into the Ubiquitin Transfer Mechanism of RING-in-between-RING Ubiquitin ligases
Ubiquitination is a posttranslational modification that regulates virtually every aspect of cellular function in eukaryotes including cell cycle progression, endocytosis, cell signaling, transcription, translation, DNA ... -
Molecular Mechanism of the Response to Hydrogen Sulfide in C. elegans
Hydrogen sulfide (H2S) has long been known as a noxious gas, however, we now know that H2S also acts an endogenous signaling molecule in humans. H2S has been shown to mediate a wide range of biological effects, ranging ... -
The neurogenic genes in Drosophila oogenesis
(1998)The Notch receptor of Drosophila and its homologues in other organisms mediate cell-cell interactions required for the correct partitioning of cell fates within equivalence groups. Genes related to Notch and other components ... -
NMR studies of the DNA-binding domain of ADR1
(1996)Nuclear magnetic resonance spectroscopy was used to study the DNA-binding domain of yeast transcription factor ADR1. A polypeptide containing the minimal DNA-binding region was overexpressed in E. coli and purified to ... -
Nonparametric Structure Models in Local Protein Conformation Sampling and Design
Protein design relies of the identification of a sequence that specifically encodes a target conformation as a folded native state. This native states is encoded by a combination of energetically favorable local and nonlocal ... -
Nuclear Architecture and Chromatin Dynamics in Development and Regeneration
The nucleus and its contents are not just static entities which offer a location and template for transcription. In both development and disease alterations in nuclear structure and/or chromatin architecture can contribute ... -
Parsing for Differences between Lck and Src Members
There are hundreds of kinases that have been identified and grouped within eukaryotic organisms and one specific family of these kinases are known as Src Family Kinases (SFKs). A unique trait about SFKs is the large ... -
Pathogen host interactions: proteomics of Influenza NEP during infection reveals an antagonistic role in the formation of tight junctions
Host-pathogen interaction networks are key to understanding the molecular mechanisms driving disease and can provide new targets for therapeutic intervention. We discovered new host factors interacting with the influenza ... -
Programmable Design of Protein Interaction Specificity and Logic Gates
The binding specificity of DNA molecules is straightforward: adenine binds thymine, and cytosine binds guanine. This simple encoding of specificity enables the binding between two DNA molecules to be modularly and ... -
Properties of Alkaline Phosphatase from Bacillus Subtilis
(1965)An increase in the activity of alkaline phosphatase was observed in an in vitro system containing soluble and particulate fractions from Bacillus Subtills (Whiteley and Oishi, 1963). In order to determine whether the ... -
Protein Complex Structure Determination Guided by Low-Resolution Cryo-Electron Microscopy Maps
Cryo-electron microscopy of protein complexes often leads to moderate resolution maps (4-8 Ã ), with visible secondary structure elements but poorly resolved loops, making model-building challenging. In the absence of ... -
Protein design by citizen scientists
Proteins are a class of molecule best known for their tendency to fold into well-defined 3-dimensional structures. The structure of a protein is determined by the sequence of amino acid units that make up the protein. Our ... -
Protein structure determination by electron diffraction of protein microcrystals
Crystallographic methods for protein structure determination are well established and have provided valuable insight into the workings of the biological world. X-ray crystallography of three dimensional crystals has ...