Browsing Biological chemistry by Title
Now showing items 80-99 of 122
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Parsing for Differences between Lck and Src Members
There are hundreds of kinases that have been identified and grouped within eukaryotic organisms and one specific family of these kinases are known as Src Family Kinases (SFKs). A unique trait about SFKs is the large ... -
Pathogen host interactions: proteomics of Influenza NEP during infection reveals an antagonistic role in the formation of tight junctions
Host-pathogen interaction networks are key to understanding the molecular mechanisms driving disease and can provide new targets for therapeutic intervention. We discovered new host factors interacting with the influenza ... -
Programmable Design of Protein Interaction Specificity and Logic Gates
The binding specificity of DNA molecules is straightforward: adenine binds thymine, and cytosine binds guanine. This simple encoding of specificity enables the binding between two DNA molecules to be modularly and ... -
Properties of Alkaline Phosphatase from Bacillus Subtilis
(1965)An increase in the activity of alkaline phosphatase was observed in an in vitro system containing soluble and particulate fractions from Bacillus Subtills (Whiteley and Oishi, 1963). In order to determine whether the ... -
Protein Complex Structure Determination Guided by Low-Resolution Cryo-Electron Microscopy Maps
Cryo-electron microscopy of protein complexes often leads to moderate resolution maps (4-8 Ã ), with visible secondary structure elements but poorly resolved loops, making model-building challenging. In the absence of ... -
Protein design by citizen scientists
Proteins are a class of molecule best known for their tendency to fold into well-defined 3-dimensional structures. The structure of a protein is determined by the sequence of amino acid units that make up the protein. Our ... -
Protein structure determination by electron diffraction of protein microcrystals
Crystallographic methods for protein structure determination are well established and have provided valuable insight into the workings of the biological world. X-ray crystallography of three dimensional crystals has ... -
Protein Structure Determination from Cryo-electron Microscopy Density Maps
Single-particle cryo-electron microscopy (cryo-EM) has emerged as a powerful tool in structure determination of macromolecular complexes that are not suitable for crystallographic studies. Recent advances in direct electron ... -
RECQ5 promotes recombination and mutagenesis at targeted nicks through disruption of RAD51 filaments
RECQ5 mutation and overexpression have both been associated with human cancer. RECQ5 has been implicated in repair of oxidative DNA damage, a critical pathway in which inherent redundancies may mask a key role for any ... -
Regulation of microtubule nucleation and attachment to spindle pole bodies
The precise regulation and coordination of the mitotic spindle is vital for accurate chromosomal segregation within a dividing cell. The centrosome is the microtubule organizing center of the cell, responsible for nucleating ... -
Regulation of stemness in humans: the role of Hypoxia Inducible Factors and Tie2
Pluripotent stem cells hold great promise for the future of regenerative medicine. Though many studies have examined the genetic determinants of pluripotency, the role of metabolism in stemness acquisition and maintenance ... -
The Role of A-type Lamins in Normal Cardiac Function
(2013-02-25)A-type lamins, primarily represented by the proteins lamin A and lamin C, are unique intermediate filaments that localize to the inner nuclear membrane to form the nuclear lamina. The lamina helps maintain nuclear structure ... -
The role of glycogen synthase kinase 3 in early xenopus development
(1997)Experiments early in this century indicated that dorsoventral axis formation in the Xenopus embryo requires the activity of the Spemann organizer, or gastrula organizer. Cell and cytoplasmic transplantation studies ... -
Roles of the HOPS complex and Sec1/Munc18 proteins in membrane fusion
(2013-07-23)A fundamental feature of the eukaryotic cell is its diversity of membrane bound compartments. Despite a constant flux of proteins and lipids through these compartments, organelle identity and function is maintained by ... -
Running the Gamut: From Structural Biology to HIV-1 Vaccine Design
Transmembrane proteins are major drug targets in bacteria, viruses, and humans. Utilizing a combination of single-particle electron microscopy, mass spectrometry, and numerous other biophysical and biochemical techniques, ... -
Structural and computational studies of two oligonucleotide modifying enzymes: I-PpoI and T4 polynucleotide kinase
(2002)Endonucleases are a large class of enzymes that catalyze the formation of site-specific double strand breaks in DNA. Homing endonucleases are expressed from open reading frames within intervening sequences (introns or ... -
Structural and Functional Characterization of Non-Canonical Ubiquitin Conjugating Enzymes
Thirty-five years ago, during its initial discovery, the field of ubiquitination could not have been more aptly named. In fact, as the study of ubiquitin (Ub) expands, it is becoming clear that this small post-translational ... -
Structural and Functional Characterization of Ube2H, a Ubiquitin-Conjugating Enzyme with a C-terminal Extension
The human proteome contains over forty ubiquitin-conjugating enzymes, each harboring a conserved core domain. Despite their structural similarities, E2s must discriminate between hundreds of E3 ligases to interact specifically ... -
Structural and mechanistic studies of the Type IIS restriction endonuclease PaqCI and de novo designed circular tandem repeat proteins
This thesis spans the work completed on two parallel projects, to (i) study the structure and mechanism of the Type IIS restriction endonuclease PaqCI, and (ii) participate in the characterization and engineering of de ... -
Structural basis for regulation and higher order assembly of human phosphofructokinase-1
Phosphofructokinase-1 (PFK-1) is a key regulatory enzyme in the glycolytic pathway that catalyzes the ATP-dependent phosphorylation of fructose-6-phosphate (F6P) to generate fructose-1,6-bisphosphate (F16BP). The reaction ...