Browsing Biological chemistry by Subject "Molecular biology"
Now showing items 1-14 of 14
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An organelle with many jobs: Mitochondrial specialization in the retina and RPE
The eye is a complex organ with many tissue and cell types working together to facilitate vision. Photoreceptor cells in the retina initiate vision, converting light into a neuronal signal that is integrated and used to ... -
Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex
The evolutionarily conserved transcription coactivator SAGA (Spt-Ada-Gcn5 Acetyltransferase) is a multi-subunit complex with a modular structure, which has several distinct activities that are used to regulate activator-dependent ... -
De Novo Scaffold Design: From Membrane β-Barrels to ɑ-helical Repeat Oligomers
De novo design of new protein folds, shapes, and features opens up new avenues for functional design. My thesis work covers the de novo design of membrane beta barrels, important considerations for making new metal binding ... -
Designed proteins assemble antibodies into modular nanocages
Antibodies are widely used in biology and medicine, and there has been considerable interest in multivalent antibody formats that increase binding avidity and enhance signaling. However, general approaches are lacking for ... -
Insight from designing ideal αβ monomers and homo-oligomers
Previously, general principles relating secondary structure patterns to tertiary packing motifs enable design of different protein topologies stabilized by consistent local and non-local interactions. With the goal to ... -
Insights into the molecular mechanisms of mitochondrial outer membrane tethering and fusion
Mitochondrial dynamics are crucial for cellular health as perturbations in these processes which include mitochondrial fusion, division, transport and mitophagy are associated with numerous diseases, cancer and neurodegeneration. ... -
Kinetochore-microtubule coupling and its regulation during mitosis
(2014-04-30)The coupling of kinetochores to dynamic spindle microtubules is crucial for chromosome positioning and segregation, error correction, and cell cycle progression. This linkage depends on the Ndc80 complex, a conserved and ... -
Molecular Architecture and Regulation of the Budding Yeast Kinetochore-Microtubule Interface
Equal partitioning of replicated chromosomes during mitosis is vital. The genetic information stored in chromosomes provide the template for cells to produce RNA and protein molecules, which carry out cellular functions. ... -
Molecular Insights into the Ubiquitin Transfer Mechanism of RING-in-between-RING Ubiquitin ligases
Ubiquitination is a posttranslational modification that regulates virtually every aspect of cellular function in eukaryotes including cell cycle progression, endocytosis, cell signaling, transcription, translation, DNA ... -
Pathogen host interactions: proteomics of Influenza NEP during infection reveals an antagonistic role in the formation of tight junctions
Host-pathogen interaction networks are key to understanding the molecular mechanisms driving disease and can provide new targets for therapeutic intervention. We discovered new host factors interacting with the influenza ... -
The Role of A-type Lamins in Normal Cardiac Function
(2013-02-25)A-type lamins, primarily represented by the proteins lamin A and lamin C, are unique intermediate filaments that localize to the inner nuclear membrane to form the nuclear lamina. The lamina helps maintain nuclear structure ... -
Structural basis for regulation and higher order assembly of human phosphofructokinase-1
Phosphofructokinase-1 (PFK-1) is a key regulatory enzyme in the glycolytic pathway that catalyzes the ATP-dependent phosphorylation of fructose-6-phosphate (F6P) to generate fructose-1,6-bisphosphate (F16BP). The reaction ... -
The ERK MAPK pathway modulates Gq-dependent locomotion in Caenorhabditis elegans
The heterotrimeric G protein Gq regulates neuronal activity through distinct downstream effector pathways. In addition to the canonical Gq effector phospholipase C beta, the small GTPase Rho was recently identified as a ... -
Understanding cardiac metabolism through elucidation of mitochondrial protein-protein interactions
Cardiovascular disease is the leading cause of death worldwide, yet developments of new treatment has been stalled for nearly three decades and novel concepts and strategies are urgently needed. Mitochondria play a central ...