Now showing items 1-3 of 3
Development of bead injection methodology for immunoassays
The research presented in this thesis develops Bead Injection techniques for application in immunological research and with the aim of developing rapid screening methods for use in clinical settings.A method is introduced, called label dilution, which is analogous to the well-established isotope dilution method. The principle ...
T-cell signaling in response to altered myelin basic protein peptides
Experimental autoimmune encephalomyelitis (EAE) can be induced in H-2k mice with the N-terminal peptide of myelin basic protein (MBP). Helper T-cells type 1 (TH1) specific to the MBP Ac(1-11) peptide mediate the autoimmune response and it has been previously shown that the progression of EAE can be inhibited by altered peptide ...
Electron paramagnetic resonance spectroscopy of spin-labeled RNA: an emerging tool for the elucidation of RNA structure and dynamics
Electron paramagnetic resonance (EPR) spectroscopy was applied to the study of RNA structure and dynamics. A general and efficient method for the site-specific incorporation of nitroxide spin-labels into internal sites of RNA is described. The spin labeling reagent 4-isocyanato TEMPO reacts with 2'-amino modified RNA in >90% ...