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Structural and biochemical studies of the transcription termination machinery
RNA Polymerase II (PolII) dependent transcription of mRNAs is central to gene expression throughout eukaryotes. Transcription is a highly regulated process, with a defined initiation, elongation, and termination phase, all of which are controlled by multiple trans-acting protein factors and cis-acting elements on the template ...
Solid State NMR Characterization of Biosilicification Peptides
Biomimetic syntheses of useful inorganic and mineral materials are the subject of much interest in the biomineralization community, as organisms can often produce materials at ambient conditions and temperatures, as opposed to the extreme conditions of commercial methods. The focus here is on biosilicication peptides, which ...
Investigating Inactive Conformations of Protein Kinases
Protein kinases comprise a substantial fraction of the human genome and constitute a wide array of cell signaling pathways that control countless cellular processes. Improper kinase regulation has been implicated in a number of grievous diseases; hence, these enzymes have become prominent therapeutic targets. The crux of ...
Coarsening dynamics of domains in lipid membranes
We investigate isothermal diffusion and growth of micron-scale liquid domains within membranes of free-floating giant unilamellar vesicles (GUVs) with diameters between 80µm and 250µm. Domains appear after a rapid temperature quench, when the membrane is cooled through a miscibility phase transition such that coexisting ...